Hirai M, Gamou S, Kobayashi M, Shimizu N
Department of Molecular Biology, Keio University School of Medicine, Tokyo.
Jpn J Cancer Res. 1989 Mar;80(3):204-8. doi: 10.1111/j.1349-7006.1989.tb02292.x.
We analyzed protein kinase C (PKC) activity in twenty-two tumor cell lines derived from lung, pancreas, stomach, tongue and vulva, and found that lung cancer cells often (9 out of 13) exhibit significantly higher PKC activity than other types of cancer cells. The PKC in these lung cancer cells was separated into one major and one minor peaks by a Mono Q column chromatography. The PKC in the major peak had an absolute requirement for Ca2+, phosphatidylserine and 12-O-tetradecanoylphorbol-13-acetate (TPA), as expected. However, the PKC in the minor peak did not require TPA for its activation. Hydroxyapatite column chromatography revealed that the PKC in the major peak is type III. These results indicate that in lung cancer cells type III PKC activity is often elevated compared to other types of cancer cells. The growth of many lung cancer cell lines was inhibited by TPA.
我们分析了源自肺、胰腺、胃、舌和外阴的22种肿瘤细胞系中的蛋白激酶C（PKC）活性，发现肺癌细胞（13种中有9种）通常比其他类型的癌细胞表现出显著更高的PKC活性。通过Mono Q柱色谱法将这些肺癌细胞中的PKC分离为一个主峰和一个次峰。正如预期的那样，主峰中的PKC对Ca2+、磷脂酰丝氨酸和12-O-十四烷酰佛波醇-13-乙酸酯（TPA）有绝对需求。然而，次峰中的PKC激活不需要TPA。羟基磷灰石柱色谱显示主峰中的PKC是III型。这些结果表明，与其他类型的癌细胞相比，肺癌细胞中III型PKC活性通常升高。许多肺癌细胞系的生长受到TPA的抑制。
