长春新碱和阿霉素对人肺癌细胞系在甲基纤维素和悬浮培养中的克隆形成细胞的影响。
The effects of vincristine and doxorubicin on the clonogenic cells of a human lung cancer cell line in methylcellulose and suspension culture.
作者信息
Yamashita Y, Nara N, Aoki N
机构信息
First Department of Internal Medicine, Tokyo Medical and Dental University.
出版信息
Jpn J Cancer Res. 1989 Mar;80(3):277-82. doi: 10.1111/j.1349-7006.1989.tb02305.x.
The effects of vincristine (VCR) and doxorubicin (DOX) on the growth of an established line of human lung cancer cells, PC9, were studied in methylcellulose and suspension cultures. The secondary colony formation in methylcellulose and recovery of clonogenic cells in suspension were considered to reflect well the self-renewal of the clonogenic cells. When dose-response curves were obtained for VCR and DOX, the primary clonogenic cells (PE1) were more sensitive than secondary clonogenic cells (PE2) or clonogenic cells in suspension. Repeated exposure to VCR in suspension did not inhibit the exponential growth of the clonogenic cells. These data indicated that both drugs were relatively ineffective in specifically suppressing the self-renewal of the clonogenic cells.
在甲基纤维素和悬浮培养中研究了长春新碱（VCR）和阿霉素（DOX）对已建立的人肺癌细胞系PC9生长的影响。甲基纤维素中的次级集落形成和悬浮中克隆细胞的恢复被认为能很好地反映克隆细胞的自我更新。当获得VCR和DOX的剂量反应曲线时，原代克隆细胞（PE1）比次级克隆细胞（PE2）或悬浮中的克隆细胞更敏感。在悬浮中反复暴露于VCR并不抑制克隆细胞的指数生长。这些数据表明，这两种药物在特异性抑制克隆细胞的自我更新方面相对无效。
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