Karwowski J P, Jackson M, Theriault R J, Chen R H, Barlow G J, Maus M L
Anti-infective Research Division, Abbott Laboratories, Abbott Park, Illinois 60064.
J Antibiot (Tokyo). 1989 Apr;42(4):506-11. doi: 10.7164/antibiotics.42.506.
The pacidamycins are a new complex of nucleosidyl-peptide antibiotics with highly specific activity against Pseudomonas aeruginosa. They are produced by Streptomyces coeruleorubidus AB 1183F-64 which was isolated from a soil sample collected at Offenburg in the FRG. The mature spore masses of the producing organism are greenish gray to blue, and the spore chains are arranged in spirals. After the structures of the pacidamycins were determined, the fermentation medium was supplemented with component amino acids. This resulted in the directed biosynthesis of several members of the complex. The overall antibiotic recovered was increased from 1 approximately 2 mg/liter to more than 100 mg/liter through a combination of strain selection, medium manipulation and amino acid feeding experiments.
帕西达霉素是一类新的核苷基肽抗生素复合物，对铜绿假单胞菌具有高度特异性活性。它们由从联邦德国奥芬堡采集的土壤样本中分离出的天蓝色链霉菌AB 1183F - 64产生。产生该抗生素的生物体的成熟孢子团呈绿灰色至蓝色，孢子链呈螺旋状排列。在确定帕西达霉素的结构后，在发酵培养基中添加了组成氨基酸。这导致了该复合物中几个成员的定向生物合成。通过菌株筛选、培养基操作和氨基酸添加实验相结合，总的抗生素回收率从约1至2毫克/升提高到了100毫克/升以上。
