明胶红假单胞菌中一氧化碳氧化系统的膜结合
Membrane association of the carbon monoxide oxidation system in Rhodopseudomonas gelatinosa.
作者信息
Wakim B T, Uffen R L
出版信息
J Bacteriol. 1983 Jan;153(1):571-3. doi: 10.1128/jb.153.1.571-573.1983.
Abstract
A comparison of the distribution of CO oxidation activity between soluble and particulate protein fractions obtained after disruption of CO-grown Rhodopseudomonas gelatinosa 1 by French pressure cell breakage and osmotic lysis of spheroplasts suggested that, in situ, the enzyme complex was associated with the cell membrane. An improved, strictly anaerobic method is given for spectrophotometric measurement of CO oxidation activity based on the carbon monoxide:methyl viologen oxidoreductase reaction.
摘要
通过法国压榨细胞破碎法和原生质球渗透裂解对在一氧化碳环境中生长的明胶红假单胞菌1进行破碎后，比较所得可溶性和颗粒性蛋白质组分之间的一氧化碳氧化活性分布，结果表明，原位时酶复合物与细胞膜相关。基于一氧化碳：甲基紫精氧化还原酶反应，给出了一种改进的、严格厌氧的分光光度法来测定一氧化碳氧化活性。
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