黑曲霉溶血菌丝体提取物的靶向脂质分析。

Targeted lipid analysis of haemolytic mycelial extracts of Aspergillus niger.

作者信息

Novak Maruša, Sepčić Kristina, Kraševec Nada, Križaj Igor, Maček Peter, Anderluh Gregor, Guella Graziano, Mancini Ines

机构信息

Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.

National Institute of Chemistry, 1000 Ljubljana, Slovenia.

出版信息

Molecules. 2014 Jun 30;19(7):9051-69. doi: 10.3390/molecules19079051.

DOI:10.3390/molecules19079051

PMID:24983857

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6272009/

Abstract

Ethanolic extracts of mycelia from Aspergillus niger (strain N402) grown in liquid media were observed to have haemolytic activity on bovine erythrocytes. This haemolytic activity decreased significantly during the time of growth (1-3 days). Moreover, when A. niger was grown on carbon-deprived medium, the efficiency of this haemolytic activity in the ethanolic extracts was much lower than when grown in carbon-enriched medium, and became almost undetectable after 3 days of growth in carbon-deprived medium. The lipid composition of these ethanolic extracts was analysed by liquid chromatography-electrospray ionisation tandem mass spectrometry. This haemolytic activity can be mainly linked to the relative levels of the molar ratios of the unsaturated fatty acids and lysophosphatidylcholines.

摘要

观察发现，在液体培养基中生长的黑曲霉（菌株N402）菌丝体的乙醇提取物对牛红细胞具有溶血活性。在生长期间（1 - 3天），这种溶血活性显著降低。此外，当黑曲霉在碳缺乏培养基上生长时，乙醇提取物中这种溶血活性的效率远低于在碳丰富培养基上生长时的效率，并且在碳缺乏培养基中生长3天后几乎检测不到。通过液相色谱 - 电喷雾电离串联质谱法分析了这些乙醇提取物的脂质组成。这种溶血活性主要与不饱和脂肪酸和溶血磷脂酰胆碱的摩尔比的相对水平有关。