Chattopadhyay P, Banerjee S K, Sen K, Chakrabarti P
Can J Microbiol. 1985 Apr;31(4):352-5. doi: 10.1139/m85-067.
A comparative study of the mycelial lipid composition of a wild strain (V35) and one unsaturated fatty acid auxotroph (UFA2) of Aspergillus niger has been performed. The lipid composition of both strains are qualitatively the same but quantitatively different. All the strains contain the following phospholipids: cardiolipin, phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylcholine, and phosphatidylserine; and triglycerides, diglycerides, monoglycerides, ergosterol, and sterol esters as the neutral lipids; mono- and di-galactosyl diglyceride as the major glycolipids along with small amounts of the corresponding mannose analogs. Phosphatidylethanolamine and phosphatidylcholine constitute the bulk of the phospholipids. The mutant (UFA2) contains a higher level of glycerides and lower levels of sterol (both free and esterified form), phospholipids, and glycolipids than the wild type. Aspergillus niger contains C16 to C18 saturated and unsaturated fatty acids. Small amounts of long-chain (C20 to C24) and short-chain (C10 to C14) saturated and unsaturated acids are also present. Linoleic, oleic, and palmitic are the major acids, stearic and linolenic acids being minor ones. UFA2 grows only in the presence of unsaturated fatty acid (C16 or C18) and accumulates a higher concentration of supplemented acid which influences its fatty acid profile.
对黑曲霉的野生菌株（V35）和一种不饱和脂肪酸营养缺陷型菌株（UFA2）的菌丝体脂质组成进行了比较研究。两种菌株的脂质组成在定性上相同，但在定量上不同。所有菌株都含有以下磷脂：心磷脂、磷脂酰乙醇胺、磷脂酰胆碱、溶血磷脂酰乙醇胺、溶血磷脂酰胆碱和磷脂酰丝氨酸；以及甘油三酯、甘油二酯、甘油单酯、麦角固醇和固醇酯作为中性脂质；单半乳糖基甘油二酯和二半乳糖基甘油二酯作为主要糖脂，同时还有少量相应的甘露糖类似物。磷脂酰乙醇胺和磷脂酰胆碱构成了磷脂的主体。与野生型相比，突变体（UFA2）含有更高水平的甘油酯，而固醇（游离和酯化形式）、磷脂和糖脂的水平较低。黑曲霉含有C16至C18的饱和脂肪酸和不饱和脂肪酸。也存在少量长链（C20至C24）和短链（C10至C14）的饱和脂肪酸和不饱和脂肪酸。亚油酸、油酸和棕榈酸是主要的脂肪酸，硬脂酸和亚麻酸是次要的脂肪酸。UFA2仅在不饱和脂肪酸（C16或C18）存在的情况下生长，并积累较高浓度的添加酸，这会影响其脂肪酸谱。
