The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing 210093, PR China.
The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
Biosens Bioelectron. 2014 Dec 15;62:66-72. doi: 10.1016/j.bios.2014.06.033. Epub 2014 Jun 20.
Due to the intrinsic hole oxidation reaction occurred on the photoanode surface, currently developed photoelectrochemical biosensors suffer from the interference from coexisting reductive species (acting as electron donor) and a novel design strategy of photoelectrode for photoelectrochemical detection is urgently required. In this paper, a self-operating photocathode based on CdS quantum dots sensitized three-dimensional (3D) nanoporous NiO was designed and created, which showed highly selective and reversible response to dissolved oxygen (acting as electron acceptor) in the electrolyte solution. Using glucose oxidase (GOD) as a biocatalyst, a novel photoelectrochemical sensor for glucose was developed. The commonly encountered interferents such as H2O2, ascorbic acid (AA), cysteine (Cys), dopamine (DA), etc., almost had no effect for the cathodic photocurrent of the 3D NiO/CdS electrode, though these substances were proved to greatly influence the photocurrent of photoanodes, which indicated greatly improved selectivity of the method. The method was applied to detect glucose in real samples including serum and glucose injections with satisfactory results. This study could provide a new train of thought on designing of self-operating photocathode in photoelectrochemical sensing, promoting the application of semiconductor nanomaterials in photoelectrochemistry.
由于光电阳极表面发生的固有空穴氧化反应，目前开发的光电化学生物传感器受到共存还原物种（作为电子供体）的干扰，因此迫切需要光电极的新型设计策略用于光电化学检测。本文设计并构建了基于 CdS 量子点敏化三维（3D）纳米多孔 NiO 的自操作光电阴极，该光电阴极对电解质溶液中溶解氧（作为电子受体）表现出高度选择性和可逆的响应。使用葡萄糖氧化酶（GOD）作为生物催化剂，开发了一种用于葡萄糖的新型光电化学传感器。虽然已经证明这些物质会极大地影响光阳极的光电流，但常见的干扰物，如 H2O2、抗坏血酸（AA）、半胱氨酸（Cys）、多巴胺（DA）等，对 3D NiO/CdS 电极的阴极光电流几乎没有影响，这表明该方法的选择性得到了极大提高。该方法已应用于血清和葡萄糖注射液等实际样品中葡萄糖的检测，结果令人满意。本研究为光电化学传感中自操作光电阴极的设计提供了新的思路，推动了半导体纳米材料在光电化学中的应用。
