State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun 130012, China.
State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun 130012, China.
Biosens Bioelectron. 2014 Sep 15;59:350-7. doi: 10.1016/j.bios.2014.03.038. Epub 2014 Mar 29.
The ZnO inverse opal photonic crystals (IOPCs) were synthesized by the sol-gel method using the polymethylmethacrylate (PMMA) as a template. For glucose detection, glucose oxidase (GOD) was further immobilized on the inwall and surface of the IOPCs. The biosensing properties toward glucose of the Nafion/GOD/ZnO IOPCs modified FTO electrodes were carefully studied and the results indicated that the sensitivity of ZnO IOPCs modified electrode was 18 times than reference electrode due to the large surface area and uniform porous structure of ZnO IOPCs. Moreover, photoelectrochemical detection for glucose using the electrode was realized and the sensitivity approached to 52.4 µA mM(-1) cm(-2), which was about four times to electrochemical detection (14.1 µA mM(-1) cm(-2)). It indicated that photoelectrochemical detection can highly improve the sensor performance than conventional electrochemical method. It also exhibited an excellent anti-interference property and a good stability at the same time. This work provides a promising approach for realizing excellent photoelectrochemical biosensor of similar semiconductor photoelectric material.
氧化锌（ZnO）反蛋白石光子晶体（IOPCs）采用聚甲基丙烯酸甲酯（PMMA）为模板，通过溶胶-凝胶法合成。为了检测葡萄糖，进一步将葡萄糖氧化酶（GOD）固定在 IOPCs 的内壁和表面上。研究了修饰在 FTO 电极上的 Nafion/GOD/ZnO IOPCs 对葡萄糖的生物传感性能，结果表明，由于 ZnO IOPCs 的大表面积和均匀的多孔结构，修饰电极的灵敏度是参比电极的 18 倍。此外，还利用该电极实现了光电化学检测葡萄糖，其灵敏度接近 52.4 µA mM(-1) cm(-2)，约为电化学检测（14.1 µA mM(-1) cm(-2)）的四倍。这表明光电化学检测可以比传统的电化学方法显著提高传感器的性能。同时，该传感器还表现出优异的抗干扰性和良好的稳定性。这项工作为实现类似半导体光电材料的优异光电化学生物传感器提供了一种有前景的方法。
