Shen Huaqing, Hou Jianxuan, Gao Zexin, Chen Biqiang, Tan Tianwei
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
Appl Biochem Biotechnol. 2014 Aug;173(8):2279-86. doi: 10.1007/s12010-014-1033-9. Epub 2014 Jul 2.
A facile and simple one-step solvothermal method has been developed to synthesize polyethyleneimine (PEI)-modified magnetic nanoparticles. Characterization of morphology, surface charges, crystal structure, and magnetic property confirmed the efficiency of this facile synthesis route. Lipase immobilized on the PEI-modified magnetic nanoparticles was used to synthesize vitamin A palmitate from vitamin A acetate and palmitic acid. The reuse of immobilized lipase can be extended to eight times by removing water during esterification with a conversion rate above 80 % for 12 h.
已开发出一种简便的一步溶剂热法来合成聚乙烯亚胺（PEI）修饰的磁性纳米颗粒。对形态、表面电荷、晶体结构和磁性的表征证实了这种简便合成路线的有效性。固定在PEI修饰磁性纳米颗粒上的脂肪酶用于由维生素A醋酸酯和棕榈酸合成维生素A棕榈酸酯。通过在酯化过程中除水，固定化脂肪酶的重复使用次数可延长至8次，12小时内转化率高于80%。
