Thiele Todd E, Crabbe John C, Boehm Stephen L
Department of Psychology, University of North Carolina, Chapel Hill, North Carolina.
Portland Alcohol Research Center, Department of Behavioral Neuroscience, Oregon Health & Sciences University, and VA Medical Center, Portland, Oregon.
Curr Protoc Neurosci. 2014 Jul 1;68:9.49.1-9.49.12. doi: 10.1002/0471142301.ns0949s68.
One of the greatest challenges that scientists face when studying the neurobiology and/or genetics of alcohol (ethanol) consumption is that most preclinical animal models do not voluntarily consume enough ethanol to achieve pharmacologically meaningful blood ethanol concentrations (BECs). Recent rodent models have been developed that promote binge-like levels of ethanol consumption associated with high BECs (i.e., ≥100 mg/dl). This unit describes procedures for an animal model of binge-like ethanol drinking which has come to be called "drinking in the dark" (DID). The "basic" variation of DID involves replacing the water bottle with a bottle containing 20% ethanol for 2 to 4 hr, beginning 3 hr into the dark cycle, on cages of singly-housed C57BL/6J mice. Using this procedure, mice typically consume enough ethanol to achieve BECs >100 mg/dl and to exhibit behavioral evidence of intoxication. An alternative two-bottle (ethanol and water) procedure is also described.
科学家在研究酒精（乙醇）消费的神经生物学和/或遗传学过程中面临的最大挑战之一是，大多数临床前动物模型不会主动摄入足够的乙醇以达到具有药理学意义的血液乙醇浓度（BEC）。最近已开发出一些啮齿动物模型，这些模型能促进与高BEC（即≥100mg/dl）相关的类似暴饮水平的乙醇消费。本单元描述了一种类似暴饮式乙醇饮用动物模型的程序，该模型已被称为“黑暗中饮用”（DID）。DID的“基本”变体涉及在单笼饲养的C57BL/6J小鼠的笼子中，从黑暗周期开始3小时后，用装有20%乙醇的瓶子替换水瓶2至4小时。使用此程序，小鼠通常会摄入足够的乙醇以达到>100mg/dl的BEC，并表现出中毒的行为迹象。还描述了另一种双瓶（乙醇和水）程序。
