干扰素在转染病毒DNA的稳定表达系统中抑制乙型肝炎病毒复制。
Interferon inhibits hepatitis B virus replication in a stable expression system of transfected viral DNA.
作者信息
Hayashi Y, Koike K
机构信息
Department of Gene Research, Cancer Institute, Tokyo, Japan.
出版信息
J Virol. 1989 Jul;63(7):2936-40. doi: 10.1128/JVI.63.7.2936-2940.1989.
Abstract
The effect of interferon (IFN) on hepatitis B virus (HBV) replication was investigated in a stable expression system, using HepG2 cells transfected with recombinant HBV DNA. IFN was found to cause a marked reduction in the levels of both minus and plus strands of HBV DNA from core particles in the cytoplasm. Neither HBV DNA from virus particles nor the HBV surface antigen in the culture medium primarily underwent change in quantity by treatment with IFN, as was also found for HBV mRNAs and the HBV core antigen/HBV e antigen in the cytoplasm. IFN exerted no influence on HBV DNA synthesis by endogenous DNA polymerase in the core particle fraction. From these findings, it would appear that IFN inhibits HBV replication by blocking some step in the pregenome RNA-primed assembly of core particles.
摘要
在一个稳定表达系统中，利用转染了重组乙肝病毒（HBV）DNA的HepG2细胞，研究了干扰素（IFN）对乙肝病毒复制的影响。结果发现，IFN可使细胞质中核心颗粒内HBV DNA负链和正链的水平显著降低。IFN处理后，病毒颗粒中的HBV DNA以及培养基中的HBV表面抗原的量基本未发生变化，细胞质中的HBV mRNA以及HBV核心抗原/HBV e抗原也是如此。IFN对核心颗粒组分中内源性DNA聚合酶的HBV DNA合成没有影响。从这些发现来看，IFN似乎是通过阻断前基因组RNA引发的核心颗粒组装的某个步骤来抑制HBV复制的。
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