Vazirinejad Reza, Ayoobi Fateme, Arababadi Mohammad Kazemi, Eftekharian Mohammad M, Darekordi Ali, Goudarzvand Mahdi, Hassanshahi Gholamhossein, Taghavi Mohammad Mohsen, Ahmadabadi Behzad Nasiri, Kennedy Derek, Shamsizadeh Ali
Department of Physiology, Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Immunology of Infectious Diseases Research Center, Rafsanjan, Iran.
Indian J Pharmacol. 2014 May-Jun;46(3):303-8. doi: 10.4103/0253-7613.132168.
Achillea millefolium (A. millefolium) is widely used as an anti-inflammatory remedy in traditional and herbal medicine. In this study, we investigated the effect of an aqueous extract from A. millefolium on experimental autoimmune encephalomyelitis (EAE) and on the serum cytokine levels in C57BL/6 mice.
EAE was induced in 63 C57BL/6 mice weighing 20-25 g (8 weeks old). Following immunization, the treatment protocol was initiated by using different doses of an aqueous extract from A. millefolium (1, 5, and 10 mg/mouse/day). Histopathologic assessments were performed by hematoxylin and eosin (H and E) and luxol fast blue (LFB) staining. Behavioral disabilities were recorded by a camera. Serum levels of interleukin (IL)-10, IL-12, and transforming growth factor (TGF)-β were measured using enzyme-linked immunosorbent assay (ELISA).
On average, mice developed classical behavioral disabilities of EAE, 13.2 ± 1.9 days following immunization. Treatment of mice with A. millefolium led to delay the appearance of behavioral disabilities along with reduced severity of the behavioral disabilities. Treatment with A. millefolium prevented weight loss and increased serum levels of TGF-β in immunized mice with MOG35-55. EAE-induced mice, which were treated with A. millefolium, had less cerebral infiltration of inflammatory cells.
The results demonstrated that treatment with aqueous extract of A. millefolium may attenuate disease severity, inflammatory responses, and demyelinating lesions in EAE-induced mice. In addition, following treatment with A. millefolium, serum levels of TGF-βwere increased in EAE-induced mice.
蓍草在传统医学和草药医学中被广泛用作抗炎药物。在本研究中，我们调查了蓍草水提取物对实验性自身免疫性脑脊髓炎（EAE）以及C57BL/6小鼠血清细胞因子水平的影响。
将63只体重20 - 25克（8周龄）的C57BL/6小鼠诱导患上EAE。免疫后，通过使用不同剂量的蓍草水提取物（1、5和10毫克/小鼠/天）启动治疗方案。通过苏木精和伊红（H&E）染色以及Luxol固蓝（LFB）染色进行组织病理学评估。通过摄像头记录行为障碍情况。使用酶联免疫吸附测定（ELISA）测量血清白细胞介素（IL）-10、IL-12和转化生长因子（TGF）-β的水平。
平均而言，小鼠在免疫后13.2±1.9天出现典型的EAE行为障碍。用蓍草治疗小鼠导致行为障碍出现延迟，同时行为障碍的严重程度降低。用蓍草治疗可防止免疫MOG35 - 55的小鼠体重减轻，并提高其血清TGF-β水平。用蓍草治疗的EAE诱导小鼠脑内炎症细胞浸润较少。
结果表明，蓍草水提取物治疗可能减轻EAE诱导小鼠的疾病严重程度、炎症反应和脱髓鞘病变。此外，用蓍草治疗后，EAE诱导小鼠的血清TGF-β水平升高。
