Graduate School of Human Health Science, University of Nagasaki Siebold, 1-1-1 Manabino, Nagayo, Nagasaki 8512195, Japan ; Institute of Food, Nutrition and Health, Jumonji University, 2-1-28 Sugasawa, Niiza, Saitama 3528510, Japan.
Graduate School of Human Health Science, University of Nagasaki Siebold, 1-1-1 Manabino, Nagayo, Nagasaki 8512195, Japan ; Nagasaki City Hospital Organization, Nagasaki Municipal Hospital, 6-39 Shinchimachi, Nagasaki 8508555, Japan.
Gastroenterol Res Pract. 2014;2014:303184. doi: 10.1155/2014/303184. Epub 2014 Jun 2.
We hypothesized that daily intake of nondigestible saccharides delays senescence onset through the improvement of intestinal microflora. Here, we raised senescence accelerated mice prone 8 (SAMP8) on the AIN93 diet (CONT), with sucrose being substituted for 5% of fructooligosaccharide (FOS) or 5% of glucomannan (GM), 15 mice per group. Ten SAMR1 were raised as reference of normal aging with control diet. Grading of senescence was conducted using the method developed by Hosokawa, and body weight, dietary intake, and drinking water intake were measured on alternate days. Following 38 weeks of these diets we evaluated learning and memory abilities using a passive avoidance apparatus and investigated effects on the intestinal microflora, measured oxidative stress markers, and inflammatory cytokines. Continuous intake of FOS and GM significantly enhanced learning and memory ability and decelerated senescence development when compared with the CONT group. Bifidobacterium levels were significantly increased in FOS and GM-fed mice. Urinary 8OHdG, 15-isoprostane, serum TNF- α , and IL-6 were also lower in FOS-fed mice, while IL-10 in FOS and GM groups was higher than in CONT group. These findings suggest that daily intake of nondigestible saccharides delays the onset of senescence via improvement of intestinal microflora.
我们假设，每天摄入不可消化的糖类可以通过改善肠道微生物群来延缓衰老的发生。在这里，我们让衰老加速敏感 8 号（SAMP8）小鼠在AIN93 饮食（CONT）上生长，用蔗糖代替 5%的低聚果糖（FOS）或 5%的葡甘露聚糖（GM），每组 15 只小鼠。10 只 SAMR1 作为正常衰老的对照，用对照饮食喂养。衰老的分级采用 Hosokawa 开发的方法进行，每隔一天测量体重、饮食摄入量和饮水量。在这些饮食 38 周后，我们使用被动回避装置评估学习和记忆能力，并研究对肠道微生物群的影响，测量氧化应激标志物和炎症细胞因子。与 CONT 组相比，连续摄入 FOS 和 GM 显著提高了学习和记忆能力，延缓了衰老的发展。FOS 和 GM 喂养的小鼠中双歧杆菌水平显著升高。FOS 喂养的小鼠尿液 8OHdG、15-异前列腺素、血清 TNF-α和 IL-6 也较低，而 FOS 和 GM 组的 IL-10 高于 CONT 组。这些发现表明，每天摄入不可消化的糖类可以通过改善肠道微生物群来延缓衰老的发生。
