Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
J Nutr Biochem. 2020 Apr;78:108326. doi: 10.1016/j.jnutbio.2019.108326. Epub 2019 Dec 20.
This study examined the effects of a carbohydrate-restricted diet on aging, brain function, intestinal bacteria and the life span to determine long-term carbohydrate-restriction effects on the aging process in senescence-accelerated prone mice (SAMP8). Three-week-old male SAMP8 were divided into three groups after a week of preliminary feeding. One group was given a controlled diet, while the others fed on high-fat and carbohydrate-restricted diets, respectively. The mice in each group were further divided into two subgroups, of which one was the longevity measurement group. The other groups fed ad libitum until the mice were 50 weeks old. Before the test period termination, passive avoidance test evaluated the learning and memory abilities. Following the test period, serum and various mice organs were obtained and submitted for analysis. The carbohydrate-restricted diet group exhibited significant decrease in the survival rate as compared to the other two diet groups. The passive avoidance test revealed a remarkable decrease in the learning and memory ability of carbohydrate-restricted diet group as compared to the control-diet group. Measurement of lipid peroxide level in tissues displayed a marked increase in the brain and spleen of carbohydrate-restricted diet group than the control-diet and high-fat diet groups. Furthermore, notable serum IL-6 and IL-1β level (inflammation indicators) elevations, decrease in Enterobacteria (with anti-inflammatory action), increase in inflammation-inducing Enterobacteria and lowering of short-chain fatty acids levels in cecum were observed in the carbohydrate-restricted diet group. Hence, carbohydrate-restricted diet was revealed to promote aging and shortening of life in SAMP8.
本研究旨在探讨低碳水化合物饮食对衰老、大脑功能、肠道细菌和寿命的影响，以确定长期低碳水化合物限制对快速老化 SAMP8 小鼠（SAMP8）衰老过程的影响。3 周龄雄性 SAMP8 在初步喂养一周后分为三组。一组给予对照饮食，其余两组分别给予高脂肪和低碳水化合物限制饮食。每组中的小鼠进一步分为两个亚组，其中一个是长寿测量组。其他组自由进食，直到 50 周龄。在测试期结束前，进行被动回避测试以评估学习和记忆能力。测试期结束后，获得血清和各种小鼠器官进行分析。与其他两组饮食相比，低碳水化合物饮食组的存活率显著下降。与对照组相比，低碳水化合物饮食组的被动回避测试显示出明显的学习和记忆能力下降。组织脂质过氧化物水平的测量显示，低碳水化合物饮食组的大脑和脾脏中的脂质过氧化物水平明显高于对照组和高脂肪饮食组。此外，在低碳水化合物饮食组中观察到显著的血清 IL-6 和 IL-1β 水平升高（炎症指标），抗炎作用的 Enterobacteria 减少，炎症诱导的 Enterobacteria 增加，盲肠中短链脂肪酸水平降低。因此，低碳水化合物饮食促进了 SAMP8 的衰老和寿命缩短。
