Guermouche Baya, Yessoufou Akadiri, Soulimane Nassima, Merzouk Hafida, Moutairou Kabirou, Hichami Aziz, Khan Naim Akhtar
Départment de Biologie, Faculté des Sciences, Université de Tlemcen, Algeria.
Obes Res. 2004 Nov;12(11):1744-53. doi: 10.1038/oby.2004.216.
We investigated the effects of a diet containing EPAX-7010, rich in PUFAs such as eicosapentaenoic acid [20:5(n-3)] and docosahexaenoic acid [22:6(n-3)], i.e., a PUFA/EPAX regimen, on T-cell activation in diabetic pregnant rats and their obese pups.
Mild hyperglycemia in pregnant rats was induced by intraperitoneal injection of streptozotocin on Day 5 of gestation. T-cell blastogenesis was assayed by using (3)H-thymidine, whereas intracellular free calcium concentrations ([Ca(2+)]i) were measured by using Fura-2 in diabetic pregnant rats and their obese offspring.
Concavalin-A-stimulated T-cell proliferation was decreased in both pregnant diabetic rats and their obese pups as compared with control animals. Feeding the PUFA/EPAX diet restored T-cell proliferation in both groups of animals. We also employed ionomycin, which at 50 nM opens calcium channels, and thapsigargin (TG), which recruits [Ca(2+)]i from endoplasmic reticulum pool. We observed that ionomycin-induced increases in [Ca(2+)]i in T-cells of diabetic mothers and obese offspring were greater than in those of control rats. Furthermore, feeding PUFA/EPAX diet diminished significantly the ionomycin-evoked rise in [Ca(2+)]i in diabetic and obese animals. TG-induced increases in [Ca(2+)]i in T-cells of diabetic pregnant rats and their obese offspring were greater than in those of control rats. The feeding of the experimental diet significantly curtailed the TG-evoked increases in [Ca(2+)]i in both diabetic and obese rats.
Together, these observations provide evidence that T-cell activation and T-cell calcium signaling are altered during gestational diabetes and macrosomia. Hence, dietary fish oils, particularly eicosapentaenoic acid and docosahexaenoic acid, may restore these T-cell abnormalities.
我们研究了一种富含二十碳五烯酸[20:5(n - 3)]和二十二碳六烯酸[22:6(n - 3)]等多不饱和脂肪酸的饮食，即多不饱和脂肪酸/ EPAX方案（PUFA/EPAX方案），对糖尿病妊娠大鼠及其肥胖幼崽T细胞活化的影响。
妊娠第5天腹腔注射链脲佐菌素诱导妊娠大鼠轻度高血糖。用³H - 胸腺嘧啶测定T细胞增殖，而用Fura - 2测量糖尿病妊娠大鼠及其肥胖后代的细胞内游离钙浓度([Ca²⁺]i)。
与对照动物相比，妊娠糖尿病大鼠及其肥胖幼崽中刀豆蛋白A刺激的T细胞增殖均降低。给予PUFA/EPAX饮食可恢复两组动物的T细胞增殖。我们还使用了离子霉素（50 nM时可打开钙通道）和毒胡萝卜素（TG），后者可从内质网池中募集[Ca²⁺]i。我们观察到，离子霉素诱导的糖尿病母亲和肥胖后代T细胞中[Ca²⁺]i的增加大于对照大鼠。此外，给予PUFA/EPAX饮食可显著降低离子霉素诱发的糖尿病和肥胖动物中[Ca²⁺]i的升高。TG诱导的糖尿病妊娠大鼠及其肥胖后代T细胞中[Ca²⁺]i的增加大于对照大鼠。给予实验饮食可显著减少糖尿病和肥胖大鼠中TG诱发的[Ca²⁺]i的增加。
总之，这些观察结果提供了证据，表明妊娠期糖尿病和巨大儿期间T细胞活化和T细胞钙信号传导发生改变。因此，膳食鱼油，特别是二十碳五烯酸和二十二碳六烯酸，可能恢复这些T细胞异常。
