Schneider Ana Claudia Reis, Machado Alice Beatriz Mombach Pinheiro, de Assis Adriano Martimbianco, Hermes Djuli Milene, Schaefer Pedro Guilherme, Guizzo Ranieli, Fracasso Laísa Beduschi, de-Paris Fernanda, Meurer Fábio, Barth Afonso Luis, da Silveira Themis Reverbel
1 Programa de Pós-Graduação: Ciências em Gastroenterologia e Hepatologia, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
Zebrafish. 2014 Aug;11(4):371-8. doi: 10.1089/zeb.2013.0968. Epub 2014 Jul 2.
Zebrafish is a powerful tool in pharmacological research and useful to identify new therapies. Probiotics can offer therapeutic options in alcoholic liver disease. This study was done in two independent experiments: first, we confirmed the intestinal colonization of probiotic Lactobacillus rhamnosus GG (LGG) after ethanol exposure. Second, four groups were performed: control (C), probiotic (P), ethanol (E), and probiotic+ethanol (P+E). Liver histology, hepatocytes morphometry, hepatic and serum lipid quantifications were conducted in second experiment. During 4 weeks, P and P+E groups were fed with LGG supplemented feed; E and C unsupplemented. E and P+E groups received 0.5% of ethanol added into tank water. Zebrafish exposed to ethanol (E group) presented intense liver steatosis after 28 days in contrast to the almost normalized liver histology of P+E group at the same period. Liver morphometry showed a significant enlargement of hepatocytes of E group after 4 weeks (p<0.0001). Serum triglycerides decreased in P+E group compared with C, P (p<0.001), and E (p=0.004), after 14 and 28 days similarly. Serum cholesterol was also decreased by LGG; P group decreased compared with C and E after 14 days (p=0.002 and p=0.007, respectively) and P+E group decreased significantly compared with E and C groups (p<0.0001) after 28 days. Hepatic triglycerides were reduced in P+E group after 28 days compared to E (p=0.006). The persistence of LGG in zebrafish intestines was demonstrated. LGG decreased serum levels of triglycerides and cholesterol and improved hepatic steatosis.
斑马鱼是药理学研究中的有力工具，有助于确定新的治疗方法。益生菌可为酒精性肝病提供治疗选择。本研究通过两个独立实验完成：首先，我们证实了乙醇暴露后益生菌鼠李糖乳杆菌GG（LGG）在肠道的定植。其次，分为四组：对照组（C）、益生菌组（P）、乙醇组（E）和益生菌+乙醇组（P+E）。在第二个实验中进行了肝脏组织学、肝细胞形态计量学、肝脏和血清脂质定量分析。在4周内，P组和P+E组喂食添加LGG的饲料；E组和C组不添加。E组和P+E组在养殖水中添加0.5%的乙醇。与同期P+E组肝脏组织学几乎正常化相比，暴露于乙醇的斑马鱼（E组）在28天后出现严重肝脂肪变性。肝脏形态计量学显示，4周后E组肝细胞显著肿大（p<0.0001）。同样，在14天和28天后，P+E组血清甘油三酯与C组、P组（p<0.001）和E组（p=0.004）相比均下降。LGG也降低了血清胆固醇；14天后P组与C组和E组相比降低（分别为p=0.002和p=0.007），28天后P+E组与E组和C组相比显著降低（p<0.0001）。28天后，P+E组肝脏甘油三酯与E组相比降低（p=0.006）。证实了LGG在斑马鱼肠道中的持久性。LGG降低了血清甘油三酯和胆固醇水平，并改善了肝脂肪变性。
