Cakmakliogullari Elcin Kal, Sanlidag Tamer, Ersoy Betul, Akcali Sinem, Var Ahmet, Cicek Candan
From the *Department of Medical Microbiology, Karabuk State Hospital; Departments of †Medical Microbiology, ‡Pediatrics, and §Medical Biochemistry, Faculty of Medicine, Celal Bayar University, Manisa; and ∥Department of Medical Microbiology, Faculty of Medicine, Ege University, Ýzmir, Turkey.
J Investig Med. 2014 Jun;62(5):821-4. doi: 10.2310/JIM.0000000000000084.
The aims of this study were to determine the association between adenovirus-5- and adenovirus-36-specific antibodies and obesity in children and to investigate their relationship with serum lipid and leptin levels.
A cross-sectional study was performed on a total of 120 children who were divided into subgroups according to body mass index percentile as obese (≥ 95th percentile) or nonobese (<95th percentile). The presence of adenovirus-36 and adenovirus-5-neutralizing antibodies was investigated by using the serum neutralization assay. Serum leptin levels were determined by microenzyme immonoassay; high-density lipoprotein, low-density lipoprotein, triglyceride, and cholesterol levels were measured by chemiluminescence method.
The presence of adenovirus-5-specific antibodies was 28.3% and 6.6% in the obese children and in non-obese children, respectively (P = 0.02). The frequency of adenovirus-36-specific antibodies was significantly greater (P = 0.018) in the obese children (26.6%) than in the non-obese children (10.0%). Serum leptin level of the obese group were significantly higher than that of the non-obese group (P = 0.000).
Our data support the association between obesity and the presence of specific antibodies to adenovirus-36 and adenovirus-5 in children. Our research has the feature of being the first national study to indicate the relationship between adenovirus-36 and human obesity as well as the first international study to indicate the relationship between adenovirus-5 and human obesity.
本研究旨在确定儿童中腺病毒5型和腺病毒36型特异性抗体与肥胖之间的关联，并研究它们与血清脂质和瘦素水平的关系。
对总共120名儿童进行了一项横断面研究，根据体重指数百分位数将他们分为肥胖（≥第95百分位数）或非肥胖（<第95百分位数）亚组。采用血清中和试验检测腺病毒36型和腺病毒5型中和抗体的存在情况。通过微酶免疫测定法测定血清瘦素水平；采用化学发光法测量高密度脂蛋白、低密度脂蛋白、甘油三酯和胆固醇水平。
肥胖儿童中腺病毒5型特异性抗体的存在率分别为28.3%和6.6%，非肥胖儿童中为6.6%（P = 0.02）。肥胖儿童中腺病毒36型特异性抗体的频率（26.6%）显著高于非肥胖儿童（10.0%）（P = 0.018）。肥胖组的血清瘦素水平显著高于非肥胖组（P = 0.000）。
我们的数据支持儿童肥胖与腺病毒36型和腺病毒5型特异性抗体的存在之间的关联。我们的研究具有以下特点：是第一项表明腺病毒36型与人类肥胖之间关系的全国性研究，也是第一项表明腺病毒5型与人类肥胖之间关系的国际性研究。
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