Hu Yuehua, Zheng Yanhua, Wu Ya, Ni Bing, Shi Shugui
Department of Neurology, Southwest Hospital, Third Military Medical University, Institute of Interventional Cerebrovascular Disease, Chongqing 400038, China.
Department of Pathology and Experimental Medicine, 306 Hospital of PLA, Beijing 100101, China.
Mediators Inflamm. 2014;2014:813045. doi: 10.1155/2014/813045. Epub 2014 Jun 1.
Immune responses and inflammation are key elements in the pathogenesis of ischemic stroke (IS). Although the involvement of IL-17A in IS has been demonstrated using animal models, the involvement of IL-17A and IL-17-secreting T cell subsets in IS patients has not been verified, and whether the balance of Treg/IL-17-secreting T cells is altered in IS patients remains unknown. In the present study, we demonstrated that the proportion of peripheral Tregs and the levels of IL-10 and TGF- β were reduced in patients with IS compared with controls using flow cytometry (FCM), real-time PCR, and ELISA assays. However, the proportions of Th17 and γ δ T cells, the primary IL-17A-secreting cells, increased dramatically, and these effects were accompanied by increases in the levels of IL-17A, IL-23, IL-6, and IL-1 β in IS patients. These studies suggest that the increase in IL-17A-producing cells and decrease in Treg cells might contribute to the pathogenesis of IS. Manipulating the balance between Tregs and IL-17A-producing cells might be helpful for the treatment of IS.
免疫反应和炎症是缺血性中风（IS）发病机制中的关键因素。尽管使用动物模型已证明IL-17A参与IS，但IL-17A和分泌IL-17的T细胞亚群在IS患者中的参与情况尚未得到证实，并且IS患者中Treg/分泌IL-17的T细胞平衡是否改变仍不清楚。在本研究中，我们使用流式细胞术（FCM）､实时PCR和ELISA检测方法证明，与对照组相比，IS患者外周血Treg比例以及IL-10和TGF-β水平降低。然而，主要分泌IL-17A的细胞Th17和γδT细胞比例显著增加，并且这些影响伴随着IS患者中IL-17A､IL-23､IL-6和IL-1β水平的升高。这些研究表明，产生IL-17A的细胞增加和Treg细胞减少可能有助于IS的发病机制。调节Treg和产生IL-17A的细胞之间的平衡可能有助于IS的治疗。
