Pionnier Nicolas, Adamek Mikołaj, Miest Joanna J, Harris Sarah J, Matras Marek, Rakus Krzysztof Ł, Irnazarow Ilgiz, Hoole Dave
Institute of Science and Technology in Medicine, School of Life Sciences, Keele University, ST5 5BG Keele, UK.
Dis Aquat Organ. 2014 Jul 3;109(3):187-99. doi: 10.3354/dao02727.
Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a highly virulent and lethal disease of common carp Cyprinus carpio and its ornamental koi varieties. However, specific knowledge about immune mechanisms behind the infection process is very limited. We aimed to evaluate the effect of the CyHV-3 infection on the profile of 2 major components of the common carp immune acute phase response: the C-reactive protein (CRP) and the complement system. Common carp were infected with CyHV-3 by bath immersion. Fish were sampled before the infection and at 6, 12, 24, 72, 120 and 336 h post-infection for serum and head kidney, liver, gill and spleen tissues. CRP levels and complement activity were determined from the serum, whereas CRP- and complement-related genes (crp1, crp2, c1rs, bf/c2, c3, masp2) expression profiles were analysed in the tissues by quantitative PCR. Both CRP levels and complement activity increased significantly up to 10- and 3-fold, respectively, in the serum of infected fish during the challenge. Analysis revealed distinct organ- and time-dependent expression profile patterns for all selected genes. These results suggest that CRP and complement behave as acute phase reactants to CyHV-3 infection in common carp with an organ- and time-dependent response.
鲤疱疹病毒3型（CyHV - 3）是鲤（Cyprinus carpio）及其观赏锦鲤品种的一种高致病性和致死性疾病的病原体。然而，关于感染过程背后免疫机制的具体知识非常有限。我们旨在评估CyHV - 3感染对鲤免疫急性期反应的两个主要成分：C反应蛋白（CRP）和补体系统的影响。通过浸浴法用CyHV - 3感染鲤。在感染前以及感染后6、12、24、72、120和336小时采集鱼的血清以及头肾、肝脏、鳃和脾脏组织样本。从血清中测定CRP水平和补体活性，而通过定量PCR分析组织中CRP和补体相关基因（crp1、crp2、c1rs、bf/c2、c3、masp2）的表达谱。在攻毒期间，感染鱼血清中的CRP水平和补体活性分别显著升高至10倍和3倍。分析揭示了所有选定基因不同的器官和时间依赖性表达谱模式。这些结果表明，CRP和补体在鲤对CyHV - 3感染的反应中作为急性期反应物，具有器官和时间依赖性反应。
