International Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, Dorset DT4 8UB, UK.
Int J Mol Sci. 2020 Nov 11;21(22):8482. doi: 10.3390/ijms21228482.
Seroconversion and the mucosal (), (), and () and () were measured for up to 900 degree days (DD) in skin swabs from common carp exposed to koi herpesvirus (KHV or CyHV-3) at either a non-permissive temperature (12 °C) or permissive temperatures (17 and 22 °C), and in survivors subjected to temperature increase to 22 °C 500 DD after the initial exposure. The survival rate at 22 °C varied from 100% in fish initially exposed at 12 °C, to 20% at 17 °C and 0% at 22 °C. Viral shedding episodes lasted for up to 29 days (493 DD) for fish clinically infected at 17 °C, and up to 57 days (684 DD) for asymptomatic fish held at 12 °C. Up-regulation of transcripts was measured at 17 and 22 °C. Down-regulation of and transcripts was measured independent of the water temperature, followed by up-regulation after the temperature increase coinciding with seroconversion and clearance of KHV from the skin mucus. mRNA showed a negative correlation with transcripts. KHV subversion of the complement system at the mucosal site coupled with poor immunoglobulin secretion during the viral replication might contribute to the long window of viral shedding, thus facilitating viral transmission.
在非允许温度（12°C）或允许温度（17°C 和 22°C）下，将鲤鱼疱疹病毒（KHV 或 CyHV-3）暴露于锦鲤的普通鲤鱼皮肤拭子中，直至 900 个度日（DD），测量血清转化以及黏膜（）、（）、（）和（）。在最初暴露于 22°C 后，将幸存者的温度增加到 22°C 500DD。22°C 的存活率从最初在 12°C 暴露的鱼的 100%变化到 17°C 的 20%和 22°C 的 0%。在 17°C 临床感染的鱼中，病毒脱落期持续长达 29 天（493DD），在 12°C 保持无症状的鱼中持续长达 57 天（684DD）。在 17°C 和 22°C 下测量了 转录物的上调。独立于水温测量了 和 转录物的下调，随后在与血清转化和皮肤黏液中 KHV 清除一致的温度升高后上调。 mRNA 与 转录物呈负相关。在黏膜部位，KHV 颠覆补体系统，同时在病毒复制期间免疫球蛋白分泌不足，这可能导致病毒脱落的长窗口，从而促进病毒传播。
