J Tradit Chin Med. 2014 Jun;34(3):309-16. doi: 10.1016/s0254-6272(14)60095-9.
Few studies have investigated the effects produced by combinations of polysaccharides and chemotherapeutic drugs in cancer treatment. We hypothesized that a combination of polysaccharides (COP) from Lentinus edodes and Tricholoma matsutake would improve the efficacy of 5-fluorouracil (5-FU)-mediated inhibition of H22 cell growth.
Mice were injected H22 cells and then treated with either 5-FU, polysaccharides from Tricholoma matsutake (PTM), polysaccharides from Lentinus edodes (PL), PTM+PL, 5-FU+PTM, 5-FU+ PL, or 5-FU + COP. The tumor weight and volume, and splenic CD4 + and CD8 + T cell frequencies, were determined. Additionally, splenic natural killer (NK) cell and cytotoxic T lymphocyte (CTL) activities were assessed and the serum levels of tumor necrosis factor-alpha (TNF-alpha), Interleukin-2 (IL-2), and Interferon-gamma (IFN-gamma) were measured.
Compared with mice from the control, 5-FU, PL, PTM, PTM + PL, 5-FU + PL, and 5-FU + PTM groups, mice treated with 5-FU + COP showed: (a) significantly reduced tumor weight and volume (P < 0.05); (b) significantly higher serum levels of TNF-alpha, IL-2, and IFN-gamma (P < 0.05); (c) significantly increased CD4+ and CD8+ T cell frequencies in the spleen (P < 0.05); and (d) significantly increased splenic NK cell and CTL activities (P < 0.05). The tumor weight and volume in mice treated with 5-FU+PL or 5-FU+PTM were significantly reduced compared with mice treated with 5-FU alone (P < 0.05). Serum levels of TNF-alpha, IL-2, and IFN-gamma, frequencies of CD4 + and CD8+ T cells in the spleen, and splenic NK and CTL activities were also significantly increased in mice treated with 5-FU+PL or 5-FU+PTM compared with mice treated with 5-FU alone (P < 0.05).
Polysaccharides from Lentinus edodes and Tricholoma matsutake could enhance the efficacy of 5-FU-mediated H22 cell growth inhibition.
很少有研究调查多糖和化疗药物联合在癌症治疗中的作用。我们假设香菇和松口蘑多糖（COP）的组合将提高 5-氟尿嘧啶（5-FU）介导的 H22 细胞生长抑制的疗效。
向小鼠注射 H22 细胞，然后用 5-FU、松口蘑多糖（PTM）、香菇多糖（PL）、PTM+PL、5-FU+PTM、5-FU+PL 或 5-FU+COP 进行处理。测定肿瘤重量和体积，以及脾 CD4+和 CD8+T 细胞频率。此外，评估脾自然杀伤（NK）细胞和细胞毒性 T 淋巴细胞（CTL）活性，并测量肿瘤坏死因子-α（TNF-α）、白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）的血清水平。
与对照组、5-FU、PL、PTM、PTM+PL、5-FU+PL 和 5-FU+PTM 组的小鼠相比，用 5-FU+COP 处理的小鼠表现出：（a）肿瘤重量和体积明显降低（P<0.05）；（b）血清 TNF-α、IL-2 和 IFN-γ水平显著升高（P<0.05）；（c）脾 CD4+和 CD8+T 细胞频率显著升高（P<0.05）；和（d）脾 NK 细胞和 CTL 活性显著增加（P<0.05）。与单独用 5-FU 处理的小鼠相比，用 5-FU+PL 或 5-FU+PTM 处理的小鼠的肿瘤重量和体积明显降低（P<0.05）。与单独用 5-FU 处理的小鼠相比，用 5-FU+PL 或 5-FU+PTM 处理的小鼠的血清 TNF-α、IL-2 和 IFN-γ水平、脾 CD4+和 CD8+T 细胞频率以及脾 NK 和 CTL 活性也显著增加（P<0.05）。
香菇和松口蘑多糖可增强 5-FU 介导的 H22 细胞生长抑制作用。
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