Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
Int J Biol Macromol. 2018 Aug;115:994-1001. doi: 10.1016/j.ijbiomac.2018.04.094. Epub 2018 Apr 22.
This study was to investigate the physicochemical properties and anticancer effects of polysaccharides from Lentinus edodes extracted under high pressure cooking treatment (HPLPS) in vitro and in vivo. The extraction efficiency was improved. The main molecular weight of HPLPS was about 540 and about 227 kDa. And the inhibitory effects on HepG2 and HeLa cells of HPLPS were significantly increased (p < 0.05). The in vivo anticancer effect on H22 tumor bearing mice model was evaluated. The tumor growth inhibitory rate of HPLPS-H was 67.66%. The activities of ALT and AST were decreased. The activities of SOD, CAT, GSH-Px were notably increased. The expressions of IL-2 and TNF-α were increased while the expression of VEGF was decreased. These results suggested that high pressure-assisted extracted polysaccharides from Lentinus edodes might be effectively used for the treatment of hepatocellular carcinoma through its antioxidant and immunomodulatory effects.
本研究旨在探讨高压蒸煮处理（HPLPS）提取的香菇多糖的理化性质和抗癌作用，体外和体内。提高了提取效率。HPLPS 的主要分子量约为 540 和 227 kDa。HPLPS 对 HepG2 和 HeLa 细胞的抑制作用明显增强（p < 0.05）。对 H22 荷瘤小鼠模型进行体内抗癌作用评价。HPLPS-H 的肿瘤生长抑制率为 67.66%。ALT 和 AST 的活性降低。SOD、CAT、GSH-Px 的活性显著增加。IL-2 和 TNF-α 的表达增加，而 VEGF 的表达减少。这些结果表明，高压辅助提取的香菇多糖可能通过其抗氧化和免疫调节作用有效地用于治疗肝癌。
