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用于高致病性甲型禽流感（H5N1）病毒的新型灵敏诊断试剂盒的研发。

Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus.

作者信息

Tsunetsugu-Yokota Yasuko, Nishimura Kengo, Misawa Shuhei, Kobayashi-Ishihara Mie, Takahashi Hitoshi, Takayama Ikuyo, Ohnishi Kazuo, Itamura Shigeyuki, Nguyen Hang L K, Le Mai T Q, Dang Giang T, Nguyen Long T, Tashiro Masato, Kageyama Tsutomu

机构信息

Department of Immunology, National Institute of Infectious Diseases, Shinjuku Tokyo 162-8640, Japan.

出版信息

BMC Infect Dis. 2014 Jul 3;14:362. doi: 10.1186/1471-2334-14-362.

DOI:10.1186/1471-2334-14-362
PMID:24992826
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4227021/
Abstract

BACKGROUND

Sporadic emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is a serious concern because of the potential for a pandemic. Conventional or quantitative RT-PCR is the standard laboratory test to detect viral influenza infections. However, this technology requires well-equipped laboratories and highly trained personnel. A rapid, sensitive, and specific alternative screening method is needed.

METHODS

By a luminescence-linked enzyme immunoassay, we have developed a H5N1 HPAI virus detection kit using anti-H5 hemagglutinin monoclonal antibodies in combination with the detection of a universal NP antigen of the type A influenza virus. The process takes 15 minutes by use of the fully automated luminescence analyzer, POCube.

RESUTLS

We tested this H5/A kit using 19 clinical specimens from 13 patients stored in Vietnam who were infected with clade 1.1 or clade 2.3.4 H5N1 HPAI virus. Approximately 80% of clinical specimens were H5-positive using the POCube system, whereas only 10% of the H5-positive samples were detected as influenza A-positive by an immunochromatography-based rapid diagnostic kit.

CONCLUSIONS

This novel H5/A kit using POCube is served as a rapid and sensitive screening test for H5N1 HPAI virus infection in humans.

摘要

背景

高致病性禽流感（HPAI）H5N1病毒在人类中偶发感染，因其可能引发大流行而备受关注。传统或定量逆转录聚合酶链反应（RT-PCR）是检测病毒性流感感染的标准实验室检测方法。然而，该技术需要设备精良的实验室和训练有素的人员。因此需要一种快速、灵敏且特异的替代筛查方法。

方法

通过发光连接酶免疫测定法，我们开发了一种H5N1高致病性禽流感病毒检测试剂盒，该试剂盒使用抗H5血凝素单克隆抗体，并结合检测甲型流感病毒的通用核蛋白（NP）抗原。使用全自动发光分析仪POCube进行该检测过程只需15分钟。

结果

我们使用来自越南存储的13名感染1.1分支或2.3.4分支H5N1高致病性禽流感病毒患者的19份临床标本对该H5/A试剂盒进行了测试。使用POCube系统时，约80%的临床标本H5呈阳性，而基于免疫层析的快速诊断试剂盒仅检测出10%的H5阳性样本为甲型流感阳性。

结论

这种使用POCube的新型H5/A试剂盒可作为人类H5N1高致病性禽流感病毒感染的快速灵敏筛查检测方法。

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本文引用的文献

1
Broad cross-reactive epitopes of the H5N1 influenza virus identified by murine antibodies against the A/Vietnam/1194/2004 hemagglutinin.
PLoS One. 2014 Jun 19;9(6):e99201. doi: 10.1371/journal.pone.0099201. eCollection 2014.
2
Broad-spectrum detection of H5 subtype influenza A viruses with a new fluorescent immunochromatography system.
PLoS One. 2013 Nov 6;8(11):e76753. doi: 10.1371/journal.pone.0076753. eCollection 2013.
3
The avian and mammalian host range of highly pathogenic avian H5N1 influenza.
Virus Res. 2013 Dec 5;178(1):3-11. doi: 10.1016/j.virusres.2013.09.004. Epub 2013 Sep 8.
4
Emergence of multiple clade 2.3.2.1 influenza A (H5N1) virus subgroups in Vietnam and detection of novel reassortants.
Virology. 2013 Sep;444(1-2):12-20. doi: 10.1016/j.virol.2013.06.005. Epub 2013 Jul 10.
5
Frequency and patterns of reassortment in natural influenza A virus infection in a reservoir host.
Virology. 2013 Aug 15;443(1):150-60. doi: 10.1016/j.virol.2013.05.004. Epub 2013 May 28.
6
Newly established monoclonal antibodies for immunological detection of H5N1 influenza virus.
Jpn J Infect Dis. 2012;65(1):19-27.
7
One-step real-time reverse transcription-PCR assays for detecting and subtyping pandemic influenza A/H1N1 2009, seasonal influenza A/H1N1, and seasonal influenza A/H3N2 viruses.
J Virol Methods. 2011 Jan;171(1):156-62. doi: 10.1016/j.jviromet.2010.10.018. Epub 2010 Oct 26.
8
Ducks: the "Trojan horses" of H5N1 influenza.
Influenza Other Respir Viruses. 2009 Jul;3(4):121-8. doi: 10.1111/j.1750-2659.2009.00084.x.
9
Human infection with highly pathogenic avian influenza A (H5N1) virus: review of clinical issues.
Clin Infect Dis. 2009 Jul 15;49(2):279-90. doi: 10.1086/600035.
10
The influenza virus enigma.
Cell. 2009 Feb 6;136(3):402-10. doi: 10.1016/j.cell.2009.01.029.

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