用于定量测定巴龙霉素的液相色谱方法的开发。在体外释放和离体渗透研究中的应用。
Development of a liquid chromatographic method for the quantification of paromomycin. Application to in vitro release and ex vivo permeation studies.
作者信息
Pujol-Brugués A, Calpena-Campmany A C, Riera-Lizandra C, Halbaut-Bellowa L, Clares-Naveros B
机构信息
Department of Medical Microbiology and Parasitology, School of Pharmacy, University of Barcelona, Joan XXIII Ave., 08028 Barcelona, Spain.
Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Barcelona, Joan XXIII Ave., 08028 Barcelona, Spain.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10;133:657-62. doi: 10.1016/j.saa.2014.06.017. Epub 2014 Jun 14.
We have developed a reversed phase high performance liquid chromatography pulsed amperometric detection (RPHPLC-PAD) method for the determination of paromomycin. It is sensitive, repeatable, and selective without the pretreatment step. Trifluoroacetic acid-water was utilized as the eluent and detected by PAD under NaOH alkaline conditions. The paromomycin detection limit (S/N=3.3) was 2μgmL(-1) and the quantification limit (S/N=10) was 6μgmL(-1). Coefficients of linear regression were higher than 0.99 for concentrations between 6.25 and 200μgmL(-1). The intra and inter-day precision (RSD) was less than 6.5%. The average recoveries were 97.53-102.01%. The proposed HPLC-PAD method presented advantageous performance characteristics and it can be considered suitable for the evaluation of paromomycin loaded nanogel formulation in ex vivo permeation and in vitro release studies.
我们开发了一种反相高效液相色谱-脉冲安培检测法（RPHPLC-PAD）来测定巴龙霉素。该方法灵敏、可重复且具有选择性，无需预处理步骤。以三氟乙酸-水作为洗脱液，并在氢氧化钠碱性条件下通过脉冲安培检测法进行检测。巴龙霉素的检测限（信噪比S/N = 3.3）为2μg/mL，定量限（信噪比S/N = 10）为6μg/mL。浓度在6.25至200μg/mL之间时，线性回归系数高于0.99。日内和日间精密度（相对标准偏差RSD）小于6.5%。平均回收率为97.53 - 102.01%。所提出的高效液相色谱-脉冲安培检测法具有良好的性能特征，可认为适用于评估载有巴龙霉素的纳米凝胶制剂的体外渗透和体外释放研究。
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