Kwon Ha-Jeong, Jeong Ji-Seon, Sim Hee-Jung, Lee Yong-Moon, Kim Yeong Shik, Hong Seon-Pyo
Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
J Chromatogr A. 2009 May 15;1216(20):4445-50. doi: 10.1016/j.chroma.2009.03.031. Epub 2009 Mar 18.
We determined the minute amount of non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection (RP-HPLC-PAD) method. Non-polar ginsenosides efficiently extracted by ethyl acetate were well separated in 40min using a water-acetonitrile gradient eluent and detected by PAD under NaOH alkaline conditions. The ginsenoside detection limits (S/N=3) were 0.03-0.10ng. The coefficients of linear regression were 0.9972-0.9990. Intra- and inter-day precision (RSDs) was less than 8.34% and average recovery was 98.06-102.73%. The total amount of non-polar ginsenosides in hairy root of red ginseng was slightly higher than in the main root.
我们采用反相高效液相色谱-脉冲安培检测法（RP-HPLC-PAD）测定了红参中痕量的非极性人参皂苷。用乙酸乙酯高效萃取的非极性人参皂苷，采用水-乙腈梯度洗脱液在40分钟内得到良好分离，并在NaOH碱性条件下通过PAD进行检测。人参皂苷的检测限（S/N=3）为0.03-0.10ng。线性回归系数为0.9972-0.9990。日内和日间精密度（RSDs）小于8.34%，平均回收率为98.06-102.73%。红参毛状根中非极性人参皂苷的总量略高于主根。
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