Liu Mingying, Qiao Guirong, Jiang Jing, Han Xiaojiao, Sang Jian, Zhuo Renying
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Road, Beijing, 100091, China.
Mol Biol Rep. 2014 Oct;41(10):6555-68. doi: 10.1007/s11033-014-3539-1. Epub 2014 Jul 4.
Salt stress exerts negative effects on plant growth, development and yields, with roots being the primary site of both perception and damage. Salix matsudana (Chinese willow) is tolerant of high salinity. However, genes associated with this trait were rarely characterized. Therefore, we first performed salt-stress treatment on S. matsudana plants, then identified differentially expressed genes by comparison of salt-treated roots and untreated controls using microarray analysis. A total of 403 salt-responsive genes were identified, of which 239 were repressed and 164 were up-regulated. Functional classification analysis revealed that these genes belonged to families encoding proteins involved in metabolism, regulation of transcription, signal transduction, hormone responses, abiotic stress responses, and other processes related to growth and development. This suggested that when S. matsudana was confronted with salt stress, coordinated adjustments are made to physiological and biochemical processes, which would then allow more resources to be allocated to protective mechanisms to avoid salt injury. The expression patterns of representative genes were further validated and the diversity of the temporal profiles indicated that a combination of several genes and the initiation of diverse pathways performed functions in S. matsudana salt tolerance. This work represents the first study employing microarrays to investigate salt tolerance in S. matsudana. The data presented herein enhance our understanding of the molecular mechanisms of S. matsudana responses to salinity stress and lay the groundwork for genetic engineering strategies to improve stress tolerance of agronomically important species.
盐胁迫对植物的生长、发育和产量产生负面影响，而根系是感知和受损的主要部位。旱柳（中国柳树）耐高盐。然而，与该性状相关的基因鲜有被鉴定。因此，我们首先对旱柳植株进行盐胁迫处理，然后通过微阵列分析比较盐处理的根系和未处理的对照，鉴定差异表达基因。共鉴定出403个盐响应基因，其中239个被抑制，164个上调。功能分类分析表明，这些基因属于编码参与代谢、转录调控、信号转导、激素反应、非生物胁迫反应以及其他与生长发育相关过程的蛋白质的家族。这表明当旱柳面临盐胁迫时，其生理生化过程会进行协调调整，从而使更多资源分配到保护机制以避免盐害。代表性基因的表达模式得到进一步验证，且时间分布的多样性表明多个基因的组合以及不同途径的启动在旱柳耐盐性中发挥作用。这项工作是首次利用微阵列研究旱柳耐盐性。本文提供的数据增进了我们对旱柳对盐胁迫响应分子机制的理解，并为提高重要农艺物种胁迫耐受性的基因工程策略奠定了基础。
