Dieter P, Peters T, Schulze-Specking A, Decker K
Biochemisches Institut, Universität Freiburg, Federal Republic of Germany.
Biochem Pharmacol. 1989 May 15;38(10):1577-81. doi: 10.1016/0006-2952(89)90304-3.
Incubation of liver macrophages with zymosan, phorbol ester and calcium ionophore A 23187 led to the formation of thromboxane, prostaglandin E2 and prostaglandin D2, whereas after external addition of arachidonic acid prostaglandin E2 and prostaglandin D2 only were found. This was confirmed by the use of labeled arachidonic acid given together with the stimuli. When the liver macrophages were prelabeled with [3H]arachidonic acid, and zymosan and [14C]arachidonic acid were added simultaneously, [3H]-label only was found in thromboxane whereas both [3H]- and [14C]-labeled PGE2 and PGD2 were detected in the cell medium. These data suggest that in cultured rat liver macrophages externally added arachidonic acid is accessible to the cyclooxygenase supplying prostaglandin H2 for prostaglandin E2 and D2 synthesis but not for thromboxane synthesis.
用酵母聚糖、佛波酯和钙离子载体A 23187孵育肝巨噬细胞会导致血栓素、前列腺素E2和前列腺素D2的形成，而在外部添加花生四烯酸后，仅发现了前列腺素E2和前列腺素D2。与刺激物一起给予标记的花生四烯酸证实了这一点。当肝巨噬细胞用[3H]花生四烯酸预标记，同时加入酵母聚糖和[14C]花生四烯酸时，仅在血栓素中发现[3H]标记，而在细胞培养基中检测到[3H]和[14C]标记的前列腺素E2和前列腺素D2。这些数据表明，在培养的大鼠肝巨噬细胞中，外部添加的花生四烯酸可被环氧化酶利用，为前列腺素E2和D2的合成提供前列腺素H2，但不能用于血栓素的合成。
