Murai Masatoshi, Miyoshi Hideto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
J Bioenerg Biomembr. 2014 Aug;46(4):313-21. doi: 10.1007/s10863-014-9562-z. Epub 2014 Jul 4.
Studies on chemical modifications of bacterial and mitochondrial complex I by synthetic chemical probes as well as endogenous chemicals have provided useful information on the structural and functional aspects of this enzyme. We herein reviewed recent studies that investigated chemical modifications of complex I by endogenous chemicals (e.g. Cys-S-nitrosation, Cys-S-glutathionylation, and Ser-O-phosphorylation) and synthetic reagents (e.g. Cys-SH modification by SH-reagents and the cross-linking of nearby subunits by bifunctional cross-linkers). We also reviewed recent photoaffinity labeling studies using complex I inhibitors, which can be recognized as "site-specific modification" by synthetic chemicals. In addition, we discussed the possibility of site-specific modification by various functional probes via ligand-directed tosylate (LDT) chemistry as a promising approach for unique biophysical studies on complex I.
利用合成化学探针以及内源性化学物质对细菌和线粒体复合物I进行化学修饰的研究，为该酶的结构和功能方面提供了有用信息。我们在此回顾了最近的研究，这些研究调查了内源性化学物质（如半胱氨酸-S-亚硝化、半胱氨酸-S-谷胱甘肽化和丝氨酸-O-磷酸化）和合成试剂（如SH试剂对半胱氨酸-SH的修饰以及双功能交联剂对附近亚基的交联）对复合物I的化学修饰。我们还回顾了最近使用复合物I抑制剂的光亲和标记研究，这些抑制剂可被合成化学物质识别为“位点特异性修饰”。此外，我们讨论了通过配体导向甲苯磺酸酯（LDT）化学，利用各种功能探针进行位点特异性修饰的可能性，这是一种对复合物I进行独特生物物理研究的有前景的方法。
