Nunes Dieli Oliveira, Almenara Camila Cruz Pereira, Broseghini-Filho Gilson Brás, Silva Marito Afonso Sousa Costa, Stefanon Ivanita, Vassallo Dalton V, Padilha Alessandra S
Department of Physiological Sciences, Federal University of Espirito Santo, Av, Marechal Campos, 1468, Maruípe, 29040-091 Vitória, ES, Brazil.
Lipids Health Dis. 2014 Jul 3;13:107. doi: 10.1186/1476-511X-13-107.
Flaxseed oil has the highest concentration of omega-3 α-linolenic acid, which has been associated with cardiovascular benefit. However, the mechanism underlying the vascular effects induced through flaxseed oil is not well known. Thus, in the present study, we investigated the effects of flaxseed oil on vascular function in isolated rat aortic rings.
Wistar rats were treated daily with flaxseed oil or a control (mineral oil) intramuscular (i.m.) for fifteen days. Isolated aortic segments were used to evaluate cyclooxygenase-2 (COX-2) protein expression, superoxide anion levels and vascular reactivity experiments.
Flaxseed oil treatment increased the vasoconstrictor response of aortic rings to phenylephrine. Endothelium removal increased the response to phenylephrine in aortic segments isolated from both groups, but the effect was smaller in the treated group. L-NAME incubation similarly increased the phenylephrine response in segments from both groups. The TXA2 synthase inhibitor furegrelate, the selective COX-2 inhibitor NS 398, the TP receptor antagonist SQ 29.548, the reactive oxygen species (ROS) scavenger apocynin, the superoxide anion scavengers tiron and the phospholipase A2 inhibitor dexamethasone partially reversed the flaxseed oil-induced increase in reactivity to phenylephrine.
These findings suggest that flaxseed oil treatment increased vascular reactivity to phenylephrine through an increase in ROS production and COX-2-derived TXA2 production. The results obtained in the present study provide new insight into the effects of flaxseed oil treatment (i.m.) on vascular function.
亚麻籽油中ω-3α-亚麻酸的含量最高，其与心血管益处相关。然而，亚麻籽油诱导血管效应的潜在机制尚不清楚。因此，在本研究中，我们研究了亚麻籽油对离体大鼠主动脉环血管功能的影响。
将Wistar大鼠每天肌肉注射（i.m.）亚麻籽油或对照物（矿物油），持续15天。使用离体主动脉段评估环氧合酶-2（COX-2）蛋白表达、超氧阴离子水平和血管反应性实验。
亚麻籽油处理增加了主动脉环对去氧肾上腺素的血管收缩反应。去除内皮增加了两组离体主动脉段对去氧肾上腺素的反应，但处理组的效应较小。L-NAME孵育同样增加了两组段对去氧肾上腺素的反应。血栓素A2合酶抑制剂呋咱甲氢龙、选择性COX-2抑制剂NS 398、TP受体拮抗剂SQ 29.548、活性氧（ROS）清除剂阿朴吗啡、超氧阴离子清除剂钛铁试剂和磷脂酶A2抑制剂地塞米松部分逆转了亚麻籽油诱导的对去氧肾上腺素反应性的增加。
这些发现表明，亚麻籽油处理通过增加ROS产生和COX-2衍生的血栓素A2产生，增加了血管对去氧肾上腺素的反应性。本研究获得的结果为亚麻籽油处理（i.m.）对血管功能的影响提供了新的见解。
