大豆油可增加大鼠的 SERCA2a 表达和左心室收缩力，而不改变动脉血压。
Soybean oil increases SERCA2a expression and left ventricular contractility in rats without change in arterial blood pressure.
机构信息
Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, Brazil.
出版信息
Lipids Health Dis. 2010 May 26;9:53. doi: 10.1186/1476-511X-9-53.
BACKGROUND
Our aim was to evaluate the effects of soybean oil treatment for 15 days on arterial and ventricular pressure, myocardial mechanics and proteins involved in calcium handling.
METHODS
Wistar rats were divided in two groups receiving 100 microL of soybean oil (SB) or saline (CT) i.m. for 15 days. Ventricular performance was analyzed in male 12-weeks old Wistar rats by measuring left ventricle diastolic and systolic pressure in isolated perfused hearts according to the Langendorff technique. Protein expression was measured by Western blot analysis.
RESULTS
Systolic and diastolic arterial pressures did not differ between CT and SB rats. However, heart rate was reduced in the SB group. In the perfused hearts, left ventricular isovolumetric systolic pressure was higher in the SB hearts. The inotropic response to extracellular Ca2+ and isoproterenol was higher in the soybean-treated animals than in the control group. Myosin ATPase and Na(+)-K(+)ATPase activities, the expression of sarcoplasmic reticulum calcium pump (SERCA2a) and sodium calcium exchanger (NCX) were increased in the SB group. Although the phosfolamban (PLB) expression did not change, its phosphorylation at Ser16 was reduced while the SERCA2a/PLB ratio was increased.
CONCLUSIONS
In summary, soybean treatment for 15 days in rats increases the left ventricular performance without affecting arterial blood pressure. These changes might be associated with an increase in the myosin ATPase activity and SERCA2a expression.
背景
我们的目的是评估 15 天的大豆油处理对动脉和心室压力、心肌力学以及钙处理相关蛋白的影响。
方法
将 Wistar 大鼠分为两组，分别接受 100μL 大豆油（SB）或生理盐水（CT）肌肉注射 15 天。通过Langendorff 技术测量分离灌注心脏中的左心室舒张和收缩压，分析雄性 12 周龄 Wistar 大鼠的心室功能。通过 Western blot 分析测量蛋白质表达。
结果
CT 和 SB 大鼠的动脉收缩压和舒张压没有差异。然而，SB 组的心率降低。在灌注心脏中，SB 心脏的左心室等容收缩压更高。大豆处理动物的细胞外 Ca2+和异丙肾上腺素的变力反应高于对照组。肌球蛋白 ATP 酶和 Na(+)-K(+)ATP 酶活性、肌浆网钙泵（SERCA2a）和钠钙交换器（NCX）的表达在 SB 组中增加。虽然磷酸化酶激活蛋白（PLB）的表达没有改变，但 Ser16 位点的磷酸化减少，而 SERCA2a/PLB 比值增加。
结论
总之，大豆处理 15 天可增加大鼠的左心室功能，而不影响动脉血压。这些变化可能与肌球蛋白 ATP 酶活性和 SERCA2a 表达增加有关。
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