UMR 7266 Littoral Environnement Sociétés (LIENSs), Institut du Littoral et de l'Environnement, 2 rue Olympe de Gouges, 17000, La rochelle, France,
Environ Sci Pollut Res Int. 2014 Dec;21(24):13888-97. doi: 10.1007/s11356-014-3116-0. Epub 2014 Jul 5.
The increase of anthropogenic activities on coastal areas induces discharges of polycyclic aromatic hydrocarbons (PAHs) in aquatic ecosystem. PAH effects depend not only on their concentration and the way of contamination but also on the different developmental stages of the organism. Zebrafish were exposed to relevant concentration of pyrolytic PAHs from the first meal (i.e., 5-day post fertilization, dpf) to mature adults. Parental effect of this type of exposure was evaluated through the assessment of aerobic metabolic scope, cardiac frequency, and cardiac mRNA expression on larval and/or embryo progeny of contaminated fish. Our results suggest that cardiac frequency increased in larval descendants of fish exposed to the environmental concentration of pyrolytic PAHs (i.e., 5 ng.g(-1) of food), while a lack of effect on aerobic metabolism in 5 dpf larvae was highlighted. A surexpression of mRNA related to the cardiac calcium transporting ATPase atp2a2a, a protein essential for contraction, is in accordance with this increasing cardiac frequency. Even if cardiac development genes cmlc1 and tnnt2a were not affected at early life stages tested, complementary work on cardiac structure could be interesting to better understand PAHs action.
沿海地区人类活动的增加导致多环芳烃（PAHs）排放到水生生态系统中。PAH 的影响不仅取决于其浓度和污染方式，还取决于生物体的不同发育阶段。斑马鱼从第一餐（即受精后 5 天）到成熟成鱼时，就接触到了相关浓度的热解 PAHs。通过评估受污染鱼类的幼虫和/或胚胎后代的有氧代谢范围、心率和心脏 mRNA 表达，评估了这种暴露的母体效应。我们的结果表明，暴露于热解 PAHs 环境浓度（即 5ng.g(-1)的食物）的鱼类的幼虫后代的心率增加，而 5 dpf 幼虫的有氧代谢没有受到影响。与心脏钙转运 ATP 酶 atp2a2a 相关的 mRNA 的过度表达，该蛋白对于收缩是必需的，与这种心率增加是一致的。即使在测试的早期生命阶段，心脏发育基因 cmlc1 和 tnnt2a 没有受到影响，但对心脏结构的补充研究可能有助于更好地理解 PAHs 的作用。
