Yuan Guiping, Lu Hongke, Yin Zhongqiong, Dai Shujun, Jia Renyong, Xu Jiao, Song Xu, Li Li
Analytical & Testing Center, Sichuan University Chengdu 610064, China.
College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
Int J Clin Exp Med. 2014 May 15;7(5):1378-85. eCollection 2014.
This study aimed to determine the effects of administering a mixture of subchronic lead acetate (Pb (NO3)2) and cadmium chloride (CdCl2·2.5H2O) on the bone metabolism of rats. A control group and three experimental groups consisted of randomly selected rats. Rats in each experimental group were orally administered with a mixture of Pb (NO3)2 and CdCl2·2.5H2O with the following respective doses for 90 consecutive days: 0 mg/kg body weight b.w. (Group I, to serve as a control), 29.96 mg/kg b.w. (Group II, 29.25 + 0.71), 89.88 mg/kg b.w. (Group III, 87.74 + 2.14), and 269.65 mg/kg b.w. (Group IV, 263.23 + 6.42). Serum osteocalcin (OC) and bone-specific alkaline phosphates (BALP) were considered as bone-formation markers, whereas carboxy-terminal cross-linking telopeptides of type I collagen (CTX) in serum acted as bone resorption markers. Calcitonin (CT) and parathormone (PTH) were tested as calciotropic hormones markers. The (Ca) and phosphorus (Pi) concentrations in the serum and urine were determined. These results were indicated by a significant (P < 0.05 - P < 0.01) increase in BALP, CTX, and PTH concentrations and decrease in CT and OC concentrations. Moreover, the concentrations of Ca and Pi in the serum were decreased, whereas those in urine increased. Results indicated that the administration of Pb and Cd induced bone metabolism disorders by decreasing bone formation and increasing bone resorption to destroy the hormonal regulation of mineral metabolism as a result of Ca and Pi imbalance.
本研究旨在确定亚慢性给予醋酸铅（Pb(NO₃)₂）和氯化镉（CdCl₂·2.5H₂O）混合物对大鼠骨代谢的影响。将大鼠随机分为一个对照组和三个实验组。每个实验组的大鼠连续90天口服Pb(NO₃)₂和CdCl₂·2.5H₂O的混合物，剂量分别如下：0毫克/千克体重（I组，作为对照组）、29.96毫克/千克体重（II组，29.25 + 0.71）、89.88毫克/千克体重（III组，87.74 + 2.14）和269.65毫克/千克体重（IV组，263.23 + 6.42）。血清骨钙素（OC）和骨特异性碱性磷酸酶（BALP）被视为骨形成标志物，而血清中I型胶原羧基末端交联肽（CTX）作为骨吸收标志物。降钙素（CT）和甲状旁腺激素（PTH）作为钙调节激素标志物进行检测。测定血清和尿液中的钙（Ca）和磷（Pi）浓度。这些结果表现为BALP、CTX和PTH浓度显著升高（P < 0.05 - P < 0.01），CT和OC浓度降低。此外，血清中Ca和Pi浓度降低，而尿液中Ca和Pi浓度升高。结果表明，铅和镉的给药通过减少骨形成和增加骨吸收诱导骨代谢紊乱，由于钙和磷失衡破坏了矿物质代谢的激素调节。
