Merçot H, Massaad L
Laboratoire de Génétique des Populations Universités Paris VI & VII, France.
Heredity (Edinb). 1989 Feb;62 ( Pt 1):35-44. doi: 10.1038/hdy.1989.5.
In vitro ADH activity and ethanol tolerance were studied in males of a series of third chromosome substitution lines in Drosophila melanogaster. The lines were divided into those with a random third chromosome from a vineyard population (VO lines) and those with a selected third chromosome from males obtained after an egg-to-adult ethanol survival test on the F4 of the previous population (VE lines). Both ADH activity and ethanol tolerance varied significantly among the lines, but the characters showed no significant correlation. Ethanol tolerance (at the higher ethanol concentrations) was higher in the selected lines (VE lines) but ADH activity was not. In our lines, the in vitro ADH activity variability, linked to the regulatory genes (located on the third chromosome) and unrelated to the polymorphism of the Adh locus (located on the second chromosome), is not involved in the ethanol tolerance variability. The data suggest that in this population ethanol tolerance was acquired in nature, at least partially, by means other than increasing ADH activity.
对黑腹果蝇一系列第三染色体替换系的雄性个体进行了体外乙醇脱氢酶（ADH）活性和乙醇耐受性研究。这些品系分为两类，一类带有来自葡萄园种群的随机第三染色体（VO品系），另一类带有经过对前一种群F4代进行从卵到成虫的乙醇存活测试后从雄性个体中筛选出的第三染色体（VE品系）。品系间的ADH活性和乙醇耐受性均存在显著差异，但这些性状之间无显著相关性。在较高乙醇浓度下，筛选品系（VE品系）的乙醇耐受性较高，但ADH活性并非如此。在我们的品系中，与调控基因（位于第三染色体上）相关且与Adh基因座（位于第二染色体上）的多态性无关的体外ADH活性变异性，并不参与乙醇耐受性变异性。数据表明，在该种群中，乙醇耐受性至少部分是通过增加ADH活性以外的方式在自然条件下获得的。
