Fink A S, Irving M, Meyer J H
Department of Surgery, University of Cincinnati Medical Center, OH 45267.
Int J Pancreatol. 1989 May;4(4):443-53. doi: 10.1007/BF02938479.
Since the chain length dependency of fatty acid-induced pancreatic exocrine secretion parallels that of fatty acid-induced inhibition of gastric emptying, similar mechanisms of action may be involved. An earlier study suggested that binding of calcium might mediate fatty acid-induced inhibition of gastric emptying. This study investigated possible mediation of fatty acid-induced pancreatic secretion by calcium binding. Pancreatic secretory response to intraduodenal administration of dodecanoate and various calcium chelators (sodium EDTA, calcium-saturated EDTA, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, and sodium taurocholate) was studied in three dogs equipped with chronic pancreatic fistulae. Calcium affinity of the various test solutions was quantitated by titrating aliquots of perfusate against a standard CaCl2 solution. Sodium EDTA was found to be the most potent calcium binder (pKc 8.3); sodium dodecanoate, sodium dodecyl sulfate, and sodium dioctyl sulfosuccinate were moderate calcium binders (pKc 7.3, 7.2, 6.9, respectively), whereas sodium taurocholate and calcium-saturated EDTA were poor calcium binders. Sodium dodecanoate evoked brisk bicarbonate output (0.6-1.6 mEq/15 min). Minimal secretory responses were observed in response to all other agents tested. These findings suggest that calcium binding is not involved in mediation of fatty acid-induced pancreatic bicarbonate secretion.
由于脂肪酸诱导的胰腺外分泌分泌的链长依赖性与脂肪酸诱导的胃排空抑制的链长依赖性相似，可能涉及相似的作用机制。一项较早的研究表明，钙的结合可能介导脂肪酸诱导的胃排空抑制。本研究调查了钙结合对脂肪酸诱导的胰腺分泌的可能介导作用。在三只装有慢性胰瘘的狗身上，研究了十二指肠内给予十二烷酸盐和各种钙螯合剂（乙二胺四乙酸钠、钙饱和乙二胺四乙酸、十二烷基硫酸钠、二辛基磺基琥珀酸钠和牛磺胆酸钠）后胰腺的分泌反应。通过用标准氯化钙溶液滴定灌注液的等分试样来定量各种测试溶液的钙亲和力。发现乙二胺四乙酸钠是最有效的钙结合剂（pKc 8.3）；十二烷酸钠、十二烷基硫酸钠和二辛基磺基琥珀酸钠是中等钙结合剂（分别为pKc 7.3、7.2、6.9），而牛磺胆酸钠和钙饱和乙二胺四乙酸是较差的钙结合剂。十二烷酸钠引起快速的碳酸氢盐分泌（0.6 - 1.6 mEq/15分钟）。对所有其他测试药物观察到最小的分泌反应。这些发现表明，钙结合不参与脂肪酸诱导的胰腺碳酸氢盐分泌的介导。
