State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Biosens Bioelectron. 2014 Dec 15;62:145-50. doi: 10.1016/j.bios.2014.06.024. Epub 2014 Jun 18.
A reversible detection method for vancomycin was developed utilizing the cantilever array sensor functionalized by a designed peptide consisting of a cysteine (Cys-), a space linker (-Gly-Gly-Gly-Gly-) and a molecular recognition ligand (-L-Lys-D-Ala-D-Ala). It was found that the peptide space linker was necessary and important for the response of the cantilever array sensor. The sensing cantilevers in the array were functionalized with the peptide while the reference cantilevers were modified by 6-mercapto-1-hexanol (MCH) to eliminate the influence of environmental disturbances. The binding between vancomycin and the peptide induced a change of surface stresses in the sensing cantilevers resulting in a differential deflection between the sensing and reference cantilevers. The reciprocal of the differential deflection is linear with the reciprocal of vancomycin concentration within the range of 2 μM to 100 μM (R=0.993) at a detection limit of 0.2 μM (S/N=3). The reversible detection can be realized just by regenerating the sensing cantilevers with running buffer solution. Other antibiotics such as doxycycline, streptomycin, and kanamycin have negligible effect on the response of the sensor. The sensor can also be utilized for reversible detection of vancomycin in serum background, which clearly indicates the potential of the sensor for vancomycin detection in real biological samples.
一种基于设计的由半胱氨酸（Cys-）、空间连接子（-Gly-Gly-Gly-Gly-）和分子识别配体（-L-Lys-D-Ala-D-Ala-）组成的肽修饰的悬臂梁阵列传感器，被开发用于万古霉素的可逆检测。研究发现，肽的空间连接子对于悬臂梁阵列传感器的响应是必要和重要的。阵列中的传感悬臂梁被肽功能化，而参考悬臂梁则通过 6-巯基-1-己醇（MCH）进行修饰，以消除环境干扰的影响。万古霉素与肽的结合引起传感悬臂梁表面应力的变化，导致传感和参考悬臂梁之间产生差分挠度。差分挠度的倒数与 2 μM 至 100 μM 范围内万古霉素浓度的倒数呈线性关系（R=0.993），检测限为 0.2 μM（S/N=3）。通过用运行缓冲液溶液再生传感悬臂梁即可实现可逆检测。其他抗生素，如强力霉素、链霉素和卡那霉素，对传感器的响应几乎没有影响。该传感器还可用于血清背景中万古霉素的可逆检测，这清楚地表明该传感器在实际生物样品中检测万古霉素的潜力。
