Department of Pharmaceutical Chemistry, College of Health Sciences, University of KwaZulu Natal, Durban, 4000, South Africa.
Department of Applied Science, Women Institute of Technology, Suddhowala, Dehradun, Uttarakhand, 248007, India.
Mikrochim Acta. 2020 Jan 2;187(1):79. doi: 10.1007/s00604-019-4015-3.
A copper(II) benzene-1,3,5-tricarboxylate (BTC) metal-organic framework (MOF) was modified with poly(acrylic acid) (PAA) and then used in an electrochemical sensor for vancomycin. The MOF, synthesized via a single-pot method, has enhanced solubility and dispersibility in water as compared to HKUST-1 but without compromising its crystallinity and porosity. The MOF was placed on a glassy carbon electrode (GCE) where it shows enhanced electrocatalytic properties. This is assumed to be due to the presence of the poly(acrylic acid) that forms a network between various HKUST-1 crystals through dimer formation between the carboxy groups of BTC and PAA. This also led to better dispersion of the MOF and to improved interaction between MOF and vancomycin. The structural, spectral and electrochemical properties of the MOFs and their vancomycin complexes was characterized. The modified GCE is shown to be a viable tool for electrochemical determination (best at a working potential of 784 mV vs. Ag/AgCl) of the antibiotic vancomycin in spiked urine and serum samples. Response is linear in the 1-500 nM vancomycin concentration range, and the detection limit is 1 nM, with a relative standard deviation of ±4.3%. Graphical abstractSchematic representation of a method for determination of vancomycin. Poly(acrylic acid) modified HKUST-1 (P-HKUST-1) forms a complex with vancomycin [Van-P-HKUST-1] which is coated over glassy carbon electrode (GCE). The decrease in peak current is recorded as response to vancomycin via cyclic voltammetry.
一种铜（II）苯-1,3,5-三甲酸（BTC）金属-有机骨架（MOF）被聚（丙烯酸）（PAA）修饰，然后用于万古霉素的电化学传感器。该 MOF 通过一锅法合成，与 HKUST-1 相比，在水中的溶解度和分散性得到增强，但不影响其结晶度和孔隙率。MOF 被放置在玻碳电极（GCE）上，在那里它显示出增强的电催化性能。这被认为是由于聚（丙烯酸）的存在，通过 BTC 和 PAA 的羧基之间的二聚体形成，在各种 HKUST-1 晶体之间形成网络。这也导致 MOF 的更好分散和 MOF 与万古霉素之间的更好相互作用。MOFs 及其万古霉素配合物的结构、光谱和电化学性质进行了表征。改性 GCE 被证明是电化学测定（在 784 mV 对 Ag/AgCl 工作电位下最佳）尿液和血清样品中万古霉素的可行工具。响应在 1-500 nM 万古霉素浓度范围内呈线性，检测限为 1 nM，相对标准偏差为 ±4.3%。
