香草醛对纤维素生物质水解中纤维素酶活性的抑制作用。
Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass.
机构信息
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
出版信息
Bioresour Technol. 2014 Sep;167:324-30. doi: 10.1016/j.biortech.2014.06.035. Epub 2014 Jun 17.
Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase.
木质纤维素原料的预处理会产生多种抑制性化合物，这些化合物强烈抑制纤维素生物质的后续酶解。香草醛是一种木质素降解产物的酚类化合物。本文详细研究了香草醛对纤维素酶水解纤维素活性的影响。结果表明，香草醛在适当的浓度下可以可逆且非竞争性地抑制纤维素酶的活性，IC50 值估计为 30 g/L。利用 HCH-1 模型研究了香草醛对纤维素酶的抑制动力学，并确定了抑制常数。此外，对三种结构类似的香草醛化合物对纤维素酶活性的研究表明，香草醛的醛基和酚羟基对纤维素酶有抑制作用。这些结果为理解木质素衍生酚类化合物对纤维素酶的抑制作用提供了有价值的详细信息。
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