Tripathy N K, Patnaik K K
Department of Zoology, Berhampur University, Orissa, India.
Mutat Res. 1992 Jan;278(1):23-9. doi: 10.1016/0165-1218(92)90282-5.
Parryfos, a farm-grade formulation of monocrotophos, was tested for genotoxicity in the wing primordial cells and the male germ-line cells of Drosophila melanogaster. Larvae of the 2nd or 3rd instar, heterozygous for the wing-cell marker mutations mwh and flr3, were exposed to different concentrations of the insecticide in the food. The wings of the hatched flies were screened for the presence of mutant mosaic spots exhibiting the marker phenotypes. The frequency of induction of sex-linked recessive lethal mutations was used to assess genotoxic effects in male germ-line cells. The tested compound was genotoxic in both the somatic and the germ-line cells of Drosophila.
对硫磷是久效磷的一种农用制剂，在黑腹果蝇的翅原基细胞和雄性生殖系细胞中进行了遗传毒性测试。对于翅细胞标记突变mwh和flr3为杂合子的二龄或三龄幼虫，在食物中接触不同浓度的杀虫剂。对孵化出的果蝇翅膀进行筛选，以检测是否存在表现出标记表型的突变镶嵌斑。利用性连锁隐性致死突变的诱导频率来评估雄性生殖系细胞中的遗传毒性作用。所测试的化合物在果蝇的体细胞和生殖系细胞中均具有遗传毒性。
