对源自中国小麦花叶病毒的小RNA的分析。
Analysis of small RNAs derived from Chinese wheat mosaic virus.
作者信息
Yang Jian, Zheng Shi-Ling, Zhang Heng-Mu, Liu Xiao-Ya, Li Jing, Li Jun-Min, Chen Jian-Ping
机构信息
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
出版信息
Arch Virol. 2014 Nov;159(11):3077-82. doi: 10.1007/s00705-014-2155-7. Epub 2014 Jul 6.
The virus-derived small interfering RNAs (vsiRNAs) of Chinese wheat mosaic virus (CWMV), a member of the genus Furovirus, were characterised from wheat plants by deep sequencing. CWMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CWMV-infected wheat plants. The 5'-terminal base of vsiRNAs was biased towards A/U, suggesting that CWMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were predicted.
通过深度测序对小麦病毒属成员中国小麦花叶病毒（CWMV）的病毒衍生小干扰RNA（vsiRNAs）进行了表征。长度为21-22 nt的CWMV vsiRNAs占主导，这表明DCL2和DCL4可能参与vsiRNAs生物合成存在保守机制，并且在受CWMV感染的小麦植株中存在共同的RNA沉默途径。vsiRNAs的5'末端碱基偏向于A/U，这表明CWMV vsiRNAs可能被加载到多种含AGO的RNA诱导沉默复合体（RISC）中以干扰宿主植物的基因表达。预测了一些vsiRNAs的可能靶标。
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