Robert Marie, Zatylny-Gaudin Céline, Fournier Vincent, Corre Erwan, Le Corguillé Gildas, Bernay Benoît, Henry Joël
Université de Caen Basse-Normandie, UMR BOREA, IBFA, F-14032 Caen, France; UMR BOREA Biologie des ORganismes et Ecosystèmes Aquatiques" MNHN, UPMC, UCBN, CNRS-7208, IRD-207, France.
Aquativ (Diana Group), ZA du Gohelis, 56250 Elven, France.
J Biotechnol. 2014 Sep 30;186:30-7. doi: 10.1016/j.jbiotec.2014.06.020. Epub 2014 Jul 3.
An RNAseq approach associated to mass spectrometry was conducted to assess the composition, molecular mass distribution and primary sequence of hydrolytic peptides issued from hydrolysates of white shrimp (Litopenaeus vannamei) by-products. High performance size exclusion chromatography (HPSEC) analyses indicated that 69.2% of the 214-nm-absorbing components had apparent molecular masses below 1000 Da, and 88.3% below 2000 Da. OFFGEL-nLC-MALDI-TOF/TOF and nLC-ESI-MS/MS analyses led to the identification of 808 peptides based on the NCBI EST databank (161,397 entries) completed by the new L. vannamei databank (58,508 entries) that we created from the RNAs of tissues used for hydrolysate production. Whereas most of hydrolytic peptides have a MW below 2000 Da, preliminary investigations of antimicrobial properties revealed three antibacterial fractions that demonstrate functional activities. The abundance of small peptides as well as the biological activities detected could imply very interesting applications for shrimp hydrolysate in the field of aquaculture feeding.
采用与质谱联用的RNA测序方法，评估白虾（凡纳滨对虾）副产物水解产物中水解肽的组成、分子量分布和一级序列。高效尺寸排阻色谱（HPSEC）分析表明，在214 nm处有吸收的组分中，69.2%的表观分子量低于1000 Da，88.3%低于2000 Da。非变性凝胶-纳升液相色谱-基质辅助激光解吸电离飞行时间/串联飞行时间质谱（OFFGEL-nLC-MALDI-TOF/TOF）和纳升液相色谱-电喷雾串联质谱（nLC-ESI-MS/MS）分析基于NCBI EST数据库（161,397条记录）并结合我们从用于生产水解产物的组织RNA创建的新的凡纳滨对虾数据库（58,508条记录），鉴定出808种肽。虽然大多数水解肽的分子量低于2000 Da，但抗菌特性的初步研究发现了三种具有功能活性的抗菌组分。小肽的丰度以及检测到的生物活性表明虾水解产物在水产养殖饲料领域可能有非常有趣的应用。
