Xiang Xi, Lang Meng, Li Yan, Zhao Xia, Sun Huimin, Jiang Weiwei, Ni Ling, Song Yishan
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Shanghai 201306, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Dec 1;1186:122990. doi: 10.1016/j.jchromb.2021.122990. Epub 2021 Oct 28.
DPP-IV plays a key role for regulation of glucose metabolism in the body. The object of this study was to obtain DPP-IV inhibitors from discarded but protein-rich Penaeus vannamei (P. vannamei) head, and to explore the potential mechanism between DPP-IV and its inhibitors. P. vannamei head protein was hydrolyzed by five food grade proteases, respectively. The animal protease hydrolysate showed the highest inhibitory active. Then the hydrolysate was sequentially separated by ultrafiltration, gel filtration chromatography and reversed phase high-performance liquid chromatography (RP-HPLC), the peptides sequences were identified by LC-MS/MS and four potential peptides YPGE, VPW, HPLY, YATP showed superior DPP-IV inhibitory activity. Meanwhile, molecular docking effectively explored their mechanism through formed hydrogen bonds and hydrophobic regions. The four peptides showed better DPP-IV inhibitory activity stability with heating treatment, pH (1-10) treatment, and in vitro gastrointestinal digestion. Our results demonstrated that the protein hydrolysate from discarded P. vannamei head can be considered as a promising natural source of DPP-IV inhibitor for helping to improve glycaemic control in Type 2 diabetes.
二肽基肽酶-IV（DPP-IV）在调节机体葡萄糖代谢中起关键作用。本研究的目的是从废弃但富含蛋白质的南美白对虾头中获取DPP-IV抑制剂，并探讨DPP-IV与其抑制剂之间的潜在作用机制。分别用五种食品级蛋白酶水解南美白对虾头蛋白。动物蛋白酶水解产物显示出最高的抑制活性。然后依次通过超滤、凝胶过滤色谱和反相高效液相色谱（RP-HPLC）对水解产物进行分离，通过液相色谱-串联质谱（LC-MS/MS）鉴定肽序列，四种潜在肽YPGE、VPW、HPLY、YATP显示出优异的DPP-IV抑制活性。同时，分子对接通过形成氢键和疏水区域有效地探索了它们的作用机制。这四种肽在加热处理、pH（1-10）处理和体外胃肠道消化中显示出较好的DPP-IV抑制活性稳定性。我们的结果表明，废弃的南美白对虾头蛋白水解产物可被视为一种有前景的DPP-IV抑制剂天然来源，有助于改善2型糖尿病患者的血糖控制。
