Carrillo Wilman, Ramos Mercedes
1 Laboratory of Functional Foods, Faculty of Foods Science and Engineering, Technical University of Ambato , Ambato, Ecuador .
2 Research Institute of Food Science CIAL (CSIC-UAM) , Madrid, Spain .
J Med Food. 2018 Sep;21(9):915-926. doi: 10.1089/jmf.2017.0132. Epub 2018 Apr 24.
Hen eggs are a source of bioactive compounds, of which the hen egg white lysozyme (HEWL) protein. HEWL has a demonstrated antibacterial activity. The aim of this study was to evaluate the antimicrobial activity of native and heated HEWL hydrolysates obtained through hydrolysis with pepsin and to identify their peptides using the reversed phase high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (RP-HPLC-ESI-MS-MS) analysis. Native and heat-treated HEWL was hydrolyzed with pepsin at pH 1.2, and their antibacterial activity was tested against Escherichia coli and Staphylococcus carnosus. Two of the hydrolysates obtained presented high antibacterial activity against Gram-positive and Gram-negative bacteria. Native HEWL hydrolysate was a bactericide at 2.0 mg/mL against E. coli. Fifty-one peptide sequences were identified on the two hydrolysates. Peptides identified are cationic peptides. These peptides are rich in Lys and Arg cationic amino acids and have Trp in their sequences.
鸡蛋是生物活性化合物的来源，其中包括鸡蛋清溶菌酶（HEWL）蛋白。HEWL具有已证实的抗菌活性。本研究的目的是评估通过胃蛋白酶水解获得的天然和加热的HEWL水解产物的抗菌活性，并使用反相高效液相色谱-电喷雾电离-串联质谱（RP-HPLC-ESI-MS-MS）分析鉴定其肽段。天然和热处理的HEWL在pH 1.2条件下用胃蛋白酶水解，并测试其对大肠杆菌和肉葡萄球菌的抗菌活性。获得的两种水解产物对革兰氏阳性菌和革兰氏阴性菌均具有高抗菌活性。天然HEWL水解产物在浓度为2.0 mg/mL时对大肠杆菌具有杀菌作用。在这两种水解产物上鉴定出了51个肽序列。鉴定出的肽为阳离子肽。这些肽富含赖氨酸和精氨酸阳离子氨基酸，且序列中含有色氨酸。
