原始脊椎动物免疫球蛋白轻链基因的完整核苷酸序列。
Complete nucleotide sequence of primitive vertebrate immunoglobulin light chain genes.
作者信息
Shamblott M J, Litman G W
机构信息
Showa Research Institute, Saint Petersburg, FL 33716.
出版信息
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4684-8. doi: 10.1073/pnas.86.12.4684.
Abstract
Antibody to Heterodontus francisci (horned shark) immunoglobulin light chain was used to screen a spleen cDNA expression library, and recombinant clones encoding light chain genes were isolated. The complete sequences of the mature coding regions of two light chain genes in this phylogenetically distant vertebrate have been determined and are reported here. Comparisons of the sequences are consistent with the presence of mammalian-like framework and complementarity-determining regions. The predicted amino acid sequences of the genes are more related to mammalian lambda than to kappa light chains. The nucleotide sequences of the genes are most related to mammalian T-cell antigen receptor beta chain. Heterodontus light chain genes may reflect characteristics of the common ancestor of immunoglobulin and T-cell antigen receptors before its evolutionary diversification.
摘要
使用抗条纹斑竹鲨免疫球蛋白轻链抗体筛选脾脏cDNA表达文库，并分离出编码轻链基因的重组克隆。已确定并在此报告了这种系统发育关系较远的脊椎动物中两个轻链基因成熟编码区的完整序列。序列比较与存在类哺乳动物的框架区和互补决定区一致。这些基因预测的氨基酸序列与哺乳动物的λ链比与κ轻链更相关。这些基因的核苷酸序列与哺乳动物T细胞抗原受体β链最相关。条纹斑竹鲨轻链基因可能反映了免疫球蛋白和T细胞抗原受体共同祖先在进化分化之前的特征。
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