Litman G W, Berger L, Murphy K, Litman R, Hinds K, Erickson B W
Proc Natl Acad Sci U S A. 1985 Apr;82(7):2082-6. doi: 10.1073/pnas.82.7.2082.
A mouse VH probe has been used to identify and isolate VH homologs in a DNA library of Heterodontus francisci (horned shark). The complete nucleotide sequence of one VH gene, HXIA, has been determined and found to exhibit striking organizational homology and nucleotide identity with mammalian prototype VH genes. Metric analysis of the complete sequence is consistent with the early phylogenetic diversification of framework and complementarity-determining regions (CDR). Both the predicted amino acid sequence and the specific hybridization of the CDR2-specific, synthetic oligodeoxynucleotide probe in spleen mRNA suggest that HXIA is functionally expressed. A probe consisting of the entire coding region of this gene hybridizes with multiple components in Southern blot analysis of Heterodontus genomic DNA and together with the identification of additional unique VH+-lambda clones indicates that considerable complexity is associated with the germline VH gene family in a contemporary species that represents an early stage in the phylogenetic development of the vertebrates.
一个小鼠VH探针已被用于在皱唇鲨（角鲨）的DNA文库中鉴定和分离VH同源物。已确定一个VH基因HXIA的完整核苷酸序列，并发现其与哺乳动物原型VH基因具有显著的结构同源性和核苷酸同一性。对完整序列的度量分析与框架区和互补决定区（CDR）的早期系统发育分化一致。预测的氨基酸序列以及CDR2特异性合成寡脱氧核苷酸探针在脾脏mRNA中的特异性杂交均表明HXIA具有功能表达。由该基因的整个编码区组成的探针在皱唇鲨基因组DNA的Southern印迹分析中与多个成分杂交，并且与其他独特的VH+-λ克隆的鉴定一起表明，在代表脊椎动物系统发育发展早期阶段的当代物种中，种系VH基因家族具有相当的复杂性。
