Park Sooyeon, Park Ji-Min, Jung Yong-Taek, Lee Keun-Chul, Lee Jung-Sook, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2014 Dec;106(6):1139-46. doi: 10.1007/s10482-014-0283-4. Epub 2014 Sep 16.
A Gram-stain negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated GYSW-15(T), was isolated from a seawater of Geoje island on the South Sea, South Korea, and subjected to a polyphasic taxonomic approach. Strain GYSW-15(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain GYSW-15(T) belongs to the genus Polaribacter, clustering coherently with the type strain of Polaribacter porphyrae showing sequence similarity value of 97.0 %. Strain GYSW-15(T) exhibited 16S rRNA gene sequence similarity values of 94.3-96.4 % to the type strains of the other Polaribacter species. Strain GYSW-15(T) was found to contain MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G, iso-C16:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as the major fatty acids. The polar lipid profile of strain GYSW-15(T) containing phosphatidylethanolamine and an unidentified lipid as major components was similar to that of the type strain of P. porphyrae. The DNA G + C content of strain GYSW-15(T) was determined to be 31.2 mol% and its mean DNA-DNA relatedness with P. porphyrae LMG 26671(T) was 17 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GYSW-15(T) is separated from recognized Polaribacter species. On the basis of the data presented, strain GYSW-15(T) is considered to represent a novel species of the genus Polaribacter, for which the name Polaribacter marinivivus sp. nov. is proposed. The type strain is GYSW-15(T) ( = KCTC 42156(T) = CECT 8655(T)).
从韩国南海巨济岛的海水中分离出一株革兰氏染色阴性、无鞭毛、不滑动、需氧的杆状细菌，命名为GYSW-15(T)，并对其进行了多相分类研究。发现菌株GYSW-15(T)在30℃、pH 7.0 - 8.0和约2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株GYSW-15(T)属于极地杆菌属，与紫菜极地杆菌模式菌株聚类在一起，序列相似性值为97.0%。菌株GYSW-15(T)与其他极地杆菌属物种的模式菌株的16S rRNA基因序列相似性值为94.3 - 96.4%。发现菌株GYSW-15(T)以MK-6作为主要甲基萘醌，以异-C15:0、异-C15:1 G、异-C16:0 3-OH和总和特征3（C16:1 ω7c和/或C16:1 ω6c）作为主要脂肪酸。菌株GYSW-15(T)的极性脂质谱以磷脂酰乙醇胺和一种未鉴定的脂质为主要成分，与紫菜极地杆菌模式菌株相似。菌株GYSW-15(T)的DNA G + C含量测定为31.2 mol%，其与紫菜极地杆菌LMG 26671(T)的平均DNA-DNA相关性为17%。不同的表型特征，以及其系统发育和遗传独特性，表明菌株GYSW-15(T)与已认可的极地杆菌属物种不同。根据所提供的数据，菌株GYSW-15(T)被认为代表了极地杆菌属的一个新物种，为此提出了海洋极地杆菌新种（Polaribacter marinivivus sp. nov.）。模式菌株为GYSW-15(T)（ = KCTC 42
