Stopa Ewa, Gajęcka Magdalena, Babińska Izabella, Zielonka Lukasz, Gajęcki Maciej
Veterinary Clinic, Ewa Stopa, DVM, Dąbrowskiego, Iława, Poland.
Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego, Olsztyn, Poland.
Theriogenology. 2014 Sep 1;82(4):537-45. doi: 10.1016/j.theriogenology.2014.05.002. Epub 2014 May 10.
The experiment involved 30 clinically healthy prepubertal bitches aged approximately 70 days with an estimated initial body weight (BW) of 8 kg. The animals were randomly divided into two experimental groups (EI and EII) and a control group of 10 animals each. Group EI was administered 50 μg zearalenone (ZEN)/kg BW per os for 42 days, group EII received 75 μg zearalenone/kg BW per os for 42 days, and the control group was administered placebo per os for 42 days. The bitches were hysterectomized at the end of treatment, and samples of uterine tissue were collected for histological and morphometric analyses. The results of the study indicate that exposure to very low doses of ZEN (100% and 150% of the NOAEL) causes simple glandular hyperplasia of the endometrium accompanied by adenogenesis, angiogenesis, and vasodilatation with the related consequences. The noted changes were more pronounced in group EI and less visible in group EII in comparison with group C, which could be indicative of a hormetic dose response.
该实验涉及30只临床健康的青春期前母犬，年龄约70天，估计初始体重（BW）为8千克。将这些动物随机分为两个实验组（EI和EII）和一个对照组，每组10只动物。EI组经口给予50微克玉米赤霉烯酮（ZEN）/千克体重，持续42天；EII组经口给予75微克玉米赤霉烯酮/千克体重，持续42天；对照组经口给予安慰剂，持续42天。在治疗结束时对母犬进行子宫切除，并收集子宫组织样本进行组织学和形态计量学分析。研究结果表明，暴露于极低剂量的ZEN（无观察到有害作用水平的100%和150%）会导致子宫内膜单纯性腺体增生，并伴有腺生成、血管生成和血管舒张以及相关后果。与C组相比，EI组中观察到的变化更为明显，而EII组中则不太明显，这可能表明存在 hormetic剂量反应。
