Gajecka M, Otrocka-Domagała I
Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Pol J Vet Sci. 2013;16(1):55-62.
Companion animals, including bitches, are exposed to intoxication with zearalenone (ZEN) present in feed, and the above may lead to reproductive disorders which are pften noted in veterinary practice. The aim of this study was to determine the effect of long-term and low-dose ZEN intoxication on the immunoexpression (optical density) of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and 17beta-hydroxysteroid dehydrogenase (17beta-HSD) in the ovaries of pre-pubertal bitches. The experiment involved 30 clinically healthy, immature Beagle bitches aged approximately 70 days with initial average body weight of 8 kg. The animals were randomly divided into two experimental groups (EI and EII) and a control group (C; n = 10 in all groups). Group EI was administered 50 microg ZEN/kg BW per os over a period of 42 days, group EII received 75 microg ZEN/kg BW per os for 42 days, and group C was administered placebo per os over a period of 42 days. Weighed amounts of the analyzed mycotoxin were administered every morning in gel capsules before feeding. The animals were subjected to ovariohysterectomy at the end of the experiment. Immunocytochemical analyses were performed to localize 3beta-HSD and 17beta-HSD enzymes and determine their optical density. The results revealed that changes in the immunoexpression of the above enzymes were inversely proportional to the applied dose of ZEN substrate.
伴侣动物，包括母犬，会因饲料中存在的玉米赤霉烯酮（ZEN）而中毒，上述情况可能导致兽医实践中经常出现的生殖障碍。本研究的目的是确定长期低剂量ZEN中毒对青春期前母犬卵巢中3β-羟基类固醇脱氢酶（3β-HSD）和17β-羟基类固醇脱氢酶（17β-HSD）免疫表达（光密度）的影响。实验涉及30只临床健康、未成熟的比格母犬，年龄约70天，初始平均体重8千克。动物被随机分为两个实验组（EI和EII）和一个对照组（C；每组n = 10）。EI组在42天内口服给予50微克ZEN/千克体重，EII组在42天内口服给予75微克ZEN/千克体重，C组在42天内口服给予安慰剂。每天早上在喂食前将称好重量的分析霉菌毒素装入凝胶胶囊给药。实验结束时对动物进行卵巢子宫切除术。进行免疫细胞化学分析以定位3β-HSD和17β-HSD酶并确定其光密度。结果显示，上述酶的免疫表达变化与ZEN底物的应用剂量成反比。
