Sharma Ajitha, Shetty Manjunath, Parida Amrita, Adiga Shalini, Kamath Shobha
Department of Pharmacology, Pondicherry Institute of Medical Sciences, Puducherry, India.
Melaka Malaysian Medical College, Manipal, Karnataka, India.
Pharmacognosy Res. 2014 Jul;6(3):246-50. doi: 10.4103/0974-8490.132605.
The effects and benefits of Acacia auriculiformis on health are not well established. This study was planned to evaluate the effect of ethanolic extract of Acacia auriculiformis leaves on learning and memory in rats.
Learning and memory were evaluated using passive avoidance paradigm and rewarded alternation test (T-maze) after the oral administration of two doses (200mg/kg and 400mg/kg) of ethanolic extract of Acacia auriculiformis with rivastigmine as positive control. Forty eight rats were divided into 4 groups in each study model. Estimation of brain cholinesterase activity was done to substantiate the results of the above mentioned tests. Data was analyzed using one way Analysis of Variance (ANOVA) followed by Tukey's post-hoc test using GraphPad InStat software, version 3.06.
The extract produced a dose-dependent improvement in the memory score namely the step through latency in passive avoidance model (P < 0.001) and the percentage of correct responses in rewarded alternation test (P < 0.05). Dose-dependent inhibition of brain cholinesterase activity (P < 0.001) was also noted.
The acetylcholinesterase inhibiting property of Acacia auriculiformis contributes to its memory enhancing potential. Further large scale studies are required to elucidate its benefits on cognitive function. This may offer a promising new option for the treatment of dementia and other cognitive deficits.
耳荚相思树对健康的影响和益处尚未完全明确。本研究旨在评估耳荚相思树叶乙醇提取物对大鼠学习和记忆的影响。
以卡巴拉汀作为阳性对照，口服给予耳荚相思树叶乙醇提取物的两个剂量（200mg/kg和400mg/kg）后，使用被动回避范式和奖励交替试验（T迷宫）评估学习和记忆。在每个研究模型中，将48只大鼠分为4组。测定脑胆碱酯酶活性以证实上述试验的结果。使用GraphPad InStat软件3.06版进行单因素方差分析（ANOVA），随后进行Tukey事后检验来分析数据。
提取物在记忆评分方面产生了剂量依赖性改善，即在被动回避模型中的穿箱潜伏期（P < 0.001）以及奖励交替试验中的正确反应百分比（P <
0.05）。还观察到对脑胆碱酯酶活性的剂量依赖性抑制（P < 0.001）。
耳荚相思树的乙酰胆碱酯酶抑制特性有助于其增强记忆的潜力。需要进一步开展大规模研究以阐明其对认知功能的益处。这可能为治疗痴呆和其他认知缺陷提供一个有前景的新选择。
