Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Str. 10, 13125 Berlin, Germany.
Laboratoire de Biochimie, Centre de Biologie Humaine, Hôpital Sud, CHU Amiens, Avenue René Laënnec, 80054 Amiens and Unité INSERM U1088, Pôle Santé, Université de Picardie Jules Verne, Rue des Louvels, 80000 Amiens, France.
Fluids Barriers CNS. 2014 Jun 23;11:14. doi: 10.1186/2045-8118-11-14. eCollection 2014.
Highly abundant proteins in biological fluids such as serum or cerebrospinal fluid (CSF) can hinder the detection of proteins in lower abundance, e.g., potential biomarkers. Commercial products are available for the depletion of albumin and immunoglobulins (Igs), although most of these kits have not been validated for dog samples. The present study therefore examines the use of different types of depletion kits for dog CSF.
Three kits, with different mechanisms for the depletion of albumin and Igs, were tested with dog CSF specimens. One product significantly decreased the amount of albumin; with all kits, IgG was less efficiently removed than albumin. Mass spectrometry of the fractions eluted from the depletion columns revealed considerable co-depletion of other CSF proteins.
A commercially available depletion kit was identified which depletes albumin and (to a lower extent) immunoglobulins from dog CSF. However, the limited efficacy and the concomitant loss of other proteins from the sample should be taken into account when using this product.
在血清或脑脊液（CSF）等生物体液中，高丰度的蛋白质可能会阻碍低丰度蛋白质（例如潜在的生物标志物）的检测。市面上有用于去除白蛋白和免疫球蛋白（Igs）的商业产品，但这些试剂盒大多未经犬样本验证。本研究因此评估了不同类型的犬 CSF 去蛋白试剂盒的应用。
用犬 CSF 标本测试了三种具有不同白蛋白和 Igs 去除机制的试剂盒。一种产品可显著减少白蛋白的含量；所有试剂盒中，IgG 的去除效率均低于白蛋白。从去蛋白柱洗脱的馏分的质谱分析显示，其他 CSF 蛋白也存在相当程度的共去除。
鉴定出一种可用于犬 CSF 的商业化去蛋白试剂盒，该试剂盒可去除白蛋白和（在较低程度上）免疫球蛋白。然而，在使用该产品时，应考虑到其有限的效果以及样品中其他蛋白质的同时损失。
