Pogledic Ivana, Kostovic Ivica, Fallet-Bianco Catherine, Adle-Biassette Homa, Gressens Pierre, Verney Catherine
Inserm U676, Paris; Croatian Institute for Brain Research, Medical School, University of Zagreb, Zagreb.
Brain Pathol. 2014 Mar;24(2):128-41. doi: 10.1111/bpa.12096. Epub 2014 Feb 18.
Studies of periventricular white matter injury (PWMI) in preterm infants suggest the involvement of the transient cortical subplate zone. We studied the cortical wall of noncystic and cystic PWMI cases and controls. Non-cystic PWMI corresponded to diffuse white matter lesions, the predominant injury currently detected by imaging. Glial cell populations were analyzed in post-mortem human frontal lobes from very preterm [24–29 postconceptional weeks (pcw)] and preterm infants (30–34 pcw) using immunohistochemistry for glial fibrillary acidic protein (GFAP), monocarboxylate transporter 1(MCT1), ionized calcium-binding adapter molecule 1 (Iba1), CD68 and oligodendrocyte lineage (Olig2). Glial activation extended into the subplate in non-cystic PWMI but was restricted to the white matter in cystic PWMI. Two major age-related and laminar differences were observed in non-cystic PWMI: in very preterm cases, activated microglial cells were increased and extended into the subplate adjacent to the lesion, whereas in preterm cases, an astroglial reaction was seen not only in the subplate but throughout the cortical plate. There were no differences in Olig2-positive pre-oligodendrocytes in the subplate inPWMI cases compared with controls. The involvement of gliosis in the deep subplate supports the concept of the complex cellular vulnerability of the subplate zone during the preterm period and may explain widespread changes in magnetic resonance signal intensity in early PWMI.
对早产儿脑室周围白质损伤（PWMI）的研究表明，短暂的皮质下板层区参与其中。我们研究了非囊性和囊性PWMI病例及对照的皮质壁。非囊性PWMI对应于弥漫性白质病变，这是目前通过影像学检测到的主要损伤。使用胶质纤维酸性蛋白（GFAP）、单羧酸转运体1（MCT1）、离子钙结合衔接分子1（Iba1）、CD68和少突胶质细胞谱系（Olig2）的免疫组织化学方法，对极早产儿[孕龄24 - 29周（pcw）]和早产儿（30 - 34 pcw）的尸检人脑额叶中的胶质细胞群体进行了分析。在非囊性PWMI中，胶质细胞活化延伸至皮质下板层，但在囊性PWMI中仅限于白质。在非囊性PWMI中观察到两个主要的与年龄相关的层状差异：在极早产儿中，活化的小胶质细胞增多并延伸至病变相邻的皮质下板层，而在早产儿中，不仅在皮质下板层而且在整个皮质板层都可见星形胶质细胞反应。与对照组相比，PWMI病例皮质下板层中Olig2阳性的少突胶质前体细胞没有差异。深部皮质下板层中胶质增生的参与支持了早产儿时期皮质下板层区复杂细胞易损性的概念，并可能解释了早期PWMI中磁共振信号强度的广泛变化。
